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Instruments
WRHFlow provides access to the following instruments via PPMS.
CYTEK Aurora |
| 5 laser digital full spectrum flow cytometer with 64 fluorescent detectors and spectral un-mixing High throughput plate acquisition is to be installed in the upcoming months |
BD Symphony - SORP |
| 8 laser digital flow cytometer with 50 parameter detection with spectral un-mixing High throughput 96/384 well plate acquisition capable Options include APE Quantiflash, cascade arrays, coherent laser control, violet SSC. |
BD Fortessa - SORP |
| 5 laser digital flow cytometer with 20 parameter detection High throughput 96/384 well plate acquisition capable |
BD LSRII |  | 5 laser digital flow cytometer with 20 parameter detection High throughput 96/384 well plate acquisition capable |
BD CANTOII (x2) |  | 3 laser digital flow cytometer with 10 parameter detection Automatic tube loader and high throughput 96/384 well plate acquisition capable |
Beckman Coulter - CytoFlex |  | 2 laser digital flow cytometer (cell counter) with 4 parameter detection Violet SSC |
Imaging cell sorter - Viola |
| 3 laser digital cytometer with 10 fluorescence detectors Imaging capability on blue laser parameters 2 simultaneous sort options 96 well plate capable |
BD INFLUX |
| 5 laser digital flow cytometer with 24 parameter detection & sorting capability 6 simultaneous sort options 96 well / 384 well / aux. sort collection device Small particle detector Polarised FSC detectors Index sorting |
BD ARIAIII |  | 3 laser digital flow cytometer with 16 parameter detection & sorting capability 4 simultaneous sort options |
MILTENYIAUTOMACS PRO |
| Automated magnetic bulk cell separation |
MILTENYIGENTLEMACS |
| Automated & reproducible cell/nucleic acid extraction from tissues Heated tissue digestion |
EMD MILLIPORELUMINEX X200 |
| Multiplex analyte analysis 96 well plate in <45min Up to 100 analytes per sample (80 on MagPlex microspheres) |
ULTIMAKEREXTENDED 3+ 3D PRINTER |
| 20-200um Z resolution, 20-100oC heat bed, Print dimensions of 215x215x300mm Active levelling, Dual extrusion print heads, Materials currently available include PLA, PVA, PP, CPE, CPE+ |
BIOTEKELX50 PLATE WASHER |
| Automated 96 well plate aspiration and buffer dispenser |
SARTORIOSELECTRONIC 12-CHANNEL PIPETTE |
| 8 or 12 channel usability Stepping function Digital display microUSB charge |
BENCHMARKORBITAL SHAKER |
| 4 plate capacity Digital timer and shaking speed setting |
SEAHORSE XFe24 ANALYSER | ||
SPECTRAMAX ID5 PLATE READER |
| Absorbance, luminescence, fluorescence, spectral readings, spectral scanning readings, fluorescence lifetime. End-point, kinetic, well area scanning. Heater and shaking compatible. |
ANALYSIS PC / MAC |
| Local workstations located in C.2.34 Provides access to FlowJo |
ARGUS ANALYSIS TERMINAL x 3 |
| Provided by Sydney Informatics Hub - see here Access requires unikey registration via email to westmead.cytometry@sydney.edu.au Provides access to FlowJo (site licence required), Huygens, Matlab, Cell Profiler, ImageJ, R, RStudio and other flow/imaging software 16 vCPUs @ 2.2GHz/3.2GHz 8GB GPUs 300GB SSD / 1TB 128GB RAM #1: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, FJ (licence needed) #2: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, FJ (licence needed), Olyvia, #3: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, Olyvia, cellSens |
