CS&T troubleshooting table

Problem	Potential cause	Solution

Problem	Potential cause	Solution
PMT voltage &/or %rCV increased for one detector	PMT faulty	check PMTV history. Replace PMT if necessary

PMT voltage &/or %rCV increased for detectors on the same laser	laser out of alignment	realign laser
	laser delay setting is off	check laser delay history, check for air bubbles in waste line
	blockage	clean with wire/syringe with decon
	dirty flowcell, burnt spot on flowcell	clean flowcell by soaking with 50% decon/contrad ~15min
	Laser starting to fail	check laser power and replace laser if necessary
	Laser is off/blocked (if increase is massive)	Check if laser is on. Check if laser path is clear

PMT voltage &/or %rCV increased for detectors on multiple lasers	insufficient warmup time	allow at least 30min warmup time
	Air bubbles in the system	Check for bubbles in sheath filter/line. If bubbles are present tap on filter to remove. Run ~1L of saline through after bubbles are removed.

PMT voltage &/or %rCV increased for detectors on latter lasers	blockage or air bubble	Prime the system. Clean with wire/syringe

No events running	Blockage	clean with wire/syringe
	Instrument not pressurised properly	Check for crack on sample tube. Check tank pressurisation/tubing connections

Negative Qr value	faulty pre-amp port or board	change pre-amp port/board

High Br value	Dirty flow cell	Clean flow cell by soaking with 50% decon/contrad ~15min
	PMT faulty	Replace PMT

Notes: Violet laser on LSRII is faulty. Ignore all errors.

On LSRII red and yellow green laser share the same mirror.

For BDFACSymphony, CV for vSSC and orange laser detectors are always high. Ignore errors on these parameters