Problem | Potential cause | Solution |
|---|---|---|
PMT voltage &/or %rCV increased for one detector | laser out of alignment | realign laser |
dirty flowcell | clean flowcell | |
PMT faulty | check PMTV history. Replace PMT if necessary | |
PMT voltage &/or %rCV increased for detectors on the same laser | laser out of alignment | realign laser |
laser delay setting is off | check laser delay history, check for air bubbles in waste line | |
blockage | clean with wire/syringe with decon | |
dirty flowcell, burnt spot on flowcell | clean flowcell by soaking with 50% decon/contrad ~15min | |
Laser starting to fail | check laser power and replace laser if necessary | |
Laser is off/blocked (if increase is massive) | Check if laser is on. Check if laser path is clear | |
PMT voltage &/or %rCV increased for detectors on multiple lasers | insufficient warmup time | allow at least 30min warmup time |
Air bubbles in the system | Check for bubbles in sheath filter/line. If bubbles are present tap on filter to remove. Run ~1L of saline through after bubbles are removed. | |
PMT voltage &/or %rCV increased for detectors on latter lasers | blockage or air bubble | Prime the system. Clean with wire/syringe |
No events running | Blockage | clean with wire/syringe |
Instrument not pressurised properly | Check for crack on sample tube. Check tank pressurisation/tubing connections | |
Negative Qr value | faulty pre-amp port or board | change pre-amp port/board |
High Br value | Dirty flow cell | Clean flow cell by soaking with 50% decon/contrad ~15min |
PMT faulty | Replace PMT |
Notes: Violet laser on LSRII is faulty. Ignore all errors.
On LSRII red and yellow green laser share the same mirror.
For BDFACSymphony, CV for vSSC and orange laser detectors are always high. Ignore errors on these parameters
Add Comment