Problem | Potential cause | Solution |
|---|---|---|
PMT voltage &/or %rCV increased for one detector |
laser out of alignment
realign laser
dirty flowcell
clean flowcell
PMT faulty | check PMTV history. Replace PMT if necessary | |
PMT voltage &/or %rCV increased for detectors on the same laser | laser out of alignment | realign laser |
laser delay setting is off | check laser delay history, check for air bubbles in waste line | |
blockage | clean with wire/syringe with decon | |
dirty flowcell, burnt spot on flowcell | clean flowcell by soaking with 50% decon/contrad ~15min | |
Laser starting to fail | check laser power and replace laser if necessary | |
Laser is off/blocked (if increase is massive) | Check if laser is on. Check if laser path is clear | |
PMT voltage &/or %rCV increased for detectors on multiple lasers | insufficient warmup time | allow at least 30min warmup time |
Air bubbles in the system | Check for bubbles in sheath filter/line. If bubbles are present tap on filter to remove. Run ~1L of saline through after bubbles are removed. | |
PMT voltage &/or %rCV increased for detectors on latter lasers | blockage or air bubble | Prime the system. Clean with wire/syringe |
No events running | Blockage | clean with wire/syringe |
Instrument not pressurised properly | Check for crack on sample tube. Check tank pressurisation/tubing connections | |
Negative Qr value | faulty pre-amp port or board | change pre-amp port/board |
High Br value | Dirty flow cell | Clean flow cell by soaking with 50% decon/contrad ~15min |
PMT faulty | Replace PMT |
Notes: Violet laser on LSRII is faulty. Ignore all errors.
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