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Unstained PBMCs - RBCs lysed and fixed with BD RBC Lysis/Fixation buffer (10min @ RT, followed by 2xwashes).

Acquired at 50V intervals.

Lymphocytes gated out and data exported. 

Data is from unstained lymphocytes. Put script in same folder as .r and run. Plots should be generated in the same folder.

...

3x(median absolute deviation) of rSD @ 200V is shown. mad, is another robust indicator of SD, similar to rSD.

Script - https://github.com/westmeadcytometry/neg_rSD_plotter

...


B515_30-A650
B610_20-A650
B670_30-A700
B710_50-A650
B750_30-A500
B780_60-A600
B820_60-A500
I470_15-A600
I515_30-A500
I605_40-A600
I660_20-A550
N820_60-A500
O616_23-A600
O660_20-A650
R670_30-A650
R710_40-A650
R780_60-A550
R820_60-A450
U379_28-A500
U450_50-A450
U515_30-A500
U586_15-A700
U610_20-A600
U670_30-A600
U695_40-A650
U740_35-A500
U780_60-A600
U820_60-A500
V427_25-A550
V474_25-A500
V525_50-A500
V586_15-A600
V610_20-A600
V677_20-A650
V710_50-A500
V750_30-A450
V780_60-A550
V820_60-A450
Y586_15-A500
Y610_20-A550
Y670_30-A600
Y710_50-A550
Y780_60-A600
Y820_60-A500




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