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- Scattered signals are not displayed as they are irrelevant. Quantiflash does not emit well <400nm hence BUV395 plot is N/A & in the NIR hence why these plots look odd.
- Recorded .fcs files need to be in a subfolder called 'laser_on'.
- With this data - an optimal range of voltages can be determined. Next update will provide for minimal voltage where S/N plateaus as well as numerical deviation from linearity of the detector.
- Height parameter used - matched manual voltration in testing.
Protocol -
- New experiment, all lasers on.
- Set laser delays to 0
- Set all scaling factors to 0.5 (inc. FSC)
- Acquire data with QF scripter automatically changing the QF intensity every second . - 1000 events/second, gaussian pulse - standard settings.
- Set FSC so that all H/W FSC signals are on scale.
- Set events to record to 200k20k.
- Record data with PMT volts from 0-1000 (25V increments) - PM for app to do this unattended (vchanger. exe)
- Put data in sub folder labelled 'laser_on' and run R script.
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