Attention: Confluence is not suitable for the storage of highly confidential data. Please ensure that any data classified as Highly Protected is stored using a more secure platform.
If you have any questions, please refer to the University's data classification guide or contact ict.askcyber@sydney.edu.au

Useful Links

Owned by Joey Lai
Last updated: Oct 22, 2023

Includes:

  • qPCR basics

  • Experimental design considerations

  • Multiplexing considerations

  • Data analysis

  • Gene expression analysis 

Includes: 

  • Primer and probe design

  • qPCR optimization

  • Instrumentation

  • Troubleshooting

Includes: 

  • ddPCR basics

  • Experimental design considerations

  • Absolute quantification

  • Copy number variation analysis

  • Rare event detection

  • Data analysis

  • Troubleshooting

Includes links to:

nSolver Software (download here) and User Guides

  • nSolver User Manual
  • nSolver Quick Start Guide
  • Gene Expression Data Analysis Guidelines
    • Data QC
    • Background correction
    • Normalization
    • Assessing differential expression and fold changes
  • CNV Data Analysis Guidelines
  • Plexset Guide
  • Protein Assay Normalization and Data Analysis Guide

nSolver Advanced Analysis Module (download here)

Includes links to:

  • Advanced analysis module user manual
  • Quick Start Guide
  • SNV Analysis Guide
  • Fusion Analysis Guide

nSolver Tutorial Videos

Nextera XT Library Preparation

Coverage calculator

  • This calculator helps with determining the reagents and sequencing runs that are needed to arrive at the desired coverage for your experiment.

How do SPRI beads work?