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If you have any questions, please refer to the University's data classification guide or contact ict.askcyber@sydney.edu.au
Useful Links
- Joey Lai
Real-time PCR
Application guide
Includes:
qPCR basics
Experimental design considerations
Multiplexing considerations
Data analysis
Gene expression analysis
Technical guide
Includes:
Primer and probe design
qPCR optimization
Instrumentation
Troubleshooting
Analysis Software download links:
QX200 Digital Droplet PCR
Application guide
Includes:
ddPCR basics
Experimental design considerations
Absolute quantification
Copy number variation analysis
Rare event detection
Data analysis
Troubleshooting
Analysis software download link:
PCR primer design
- NCBI nucleotide database - search for sequences
- Primer Quest - primer design
- BLAST - Nucleotide sequence alignment
- Oligoanalyzer - Primer secondary structure analysis
Nanostring
Data analysis guides
Nanostring nSolver Resource Guide
Includes links to:
nSolver Software (download here) and User Guides
- nSolver User Manual
- nSolver Quick Start Guide
- Gene Expression Data Analysis Guidelines
- Data QC
- Background correction
- Normalization
- Assessing differential expression and fold changes
- CNV Data Analysis Guidelines
- Plexset Guide
- Protein Assay Normalization and Data Analysis Guide
nSolver Advanced Analysis Module (download here)
Includes links to:
- Advanced analysis module user manual
- Quick Start Guide
- SNV Analysis Guide
- Fusion Analysis Guide
nSolver Tutorial Videos
- Links to videos are in the nSolver Resource Guide
Next Generation Sequencing
Nextera XT Library Preparation
- This calculator helps with determining the reagents and sequencing runs that are needed to arrive at the desired coverage for your experiment.
How do SPRI beads work?
- DNA fragment size selection guide
- http://core-genomics.blogspot.com/2012/04/how-do-spri-beads-work.html
Tapestation
Best practices
Short Tandem Repeat (STR) Cell Line Authentication
- ATCC database (USA)
- DSMZ database (European)
- Cell Line Authentication Guidelines (ICLAC)