Attention: Confluence is not suitable for the storage of highly confidential data. Please ensure that any data classified as Highly Protected is stored using a more secure platform.
If you have any questions, please refer to the University's data classification guide or contact ict.askcyber@sydney.edu.au

Skip to end of banner
Go to start of banner

Instruments

Skip to end of metadata
Go to start of metadata

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 6 Next »

WRH Genomics Facility Equipment

Instruments below can be booked via the WRH PPMS website. 

InstrumentVendorImageApplications/DescriptionLocationInstrument details
C1000 Thermocycler


Biorad
               
  • PCR amplification
  • 96 well block
  • Gradient function
L2.10

Specs: http://www.bio-rad.com/en-au/product/thermal-cyclers-for-pcr/c1000-touch-thermal-cycler

Covaris Evolution UltrasonicatorCovaris

              

  • Precise DNA and RNA shearing to specified fragment lengths
  • Chromatin shearing for ChIP samples
  • DNA and RNA extraction from FFPE samples
  • Process up to 8 samples per run
J2.06

Specs: http://covarisinc.com/products/afa-ultrasonication/e220evolution/

Digital Droplet PCR System (QX200)Biorad

  • Droplet reader (left)
  • Droplet generator (right)
  • Absolute quantification of nucleic acid targets
  • Copies/ul output
  • Rare event detection
  • No standards required
  • Process up to 96 samples per run
L2.09 and L2.10

Specs: http://www.bio-rad.com/en-au/product/qx200-droplet-digital-pcr-system

Nanostring nCounter SystemNanostring

  • Expression profiling of up to 800 targets within 48 hours
  • Quantify mRNA, miRNA and protein expression
  • No cDNA synthesis necessary
  • Off-the-shelf and custom panels available
  • Process 12 samples per run
L2.10Specs: https://www.nanostring.com/application/files/2814/8970/9851/LBL-10168-01_PDS_nCounter_System.pdf
Tapestation 4200Agilent

                         

  • Quantitatively assess DNA and RNA quality 
  • RNA and DNA Integrity Numbers (RIN and DIN) are provided
  • Calculate nucleic acid concentrations and product size
  • Process up to 96 samples per run
L2.10

Specs: http://www.agilent.com/cs/library/datasheets/public/5991-6029EN.pdf

WIMR Genomics Equipment

Instruments below can be booked using the WIMR PPMS website.

InstrumentVendorImageApplications/DescriptionLocationInstrument details
CFX96 qPCR Biorad

     

  • Real time PCR
  • 96 well block
  • Gradient function
  • Detection channels:
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf
CFX384 qPCRBiorad

      

  • Real time PCR
  • 384 well block
  • Gradient function
  • Detection channels as above
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf
Stratagene MX3005PIntegrated Sciences

  • Real time PCR
  • 96 well block; semi-skirted or unskirted plates only
  • Detection channels:
    • FAM/Sybr, HEX/JOE/VIC, Alexa Fluor 350, Tet, Tamra, Cy3, ROX/Texas Red, Cy5
L2.09Specs: https://www.agilent.com/en/mx3005p-qpcr-systems-details-specifications
Rotorgene 3000

Qiagen

        

  • Real time PCR
  • Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
  • Detection channels: 4 colours
    • FAM/Sybr, JOE, ROX, Cy5
L2.09Specs: http://www.lth.se/fileadmin/sciblu/QC/RG3000.pdf
Rotorgene 6000Qiagen

  • Real time PCR
  • Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
  • Detection channels: 5 colours
    • FAM/Sybr, JOE, ROX, Cy3, Cy5, 
L2.09Specs are for Rotorgene Q (upgraded model): https://www.qiagen.com/us/shop/automated-solutions/pcr-instruments/rotor-gene-q/#orderinginformation
Veriti ThermocyclerApplied Biosystems

   

  • PCR amplification
  • 96 well block; semi-skirted or non skirted plates
L2.09Specs: https://www.thermofisher.com/au/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html
Pre-PCR hoodNuaire

  • Laminar airflow workstation
  • HEPA filtered
  • Contains UV light for decontamination
L2.11Specs: https://www.biocompare.com/19657-Laminar-Flow-Hoods-Laminar-Flow-Cabinets/73710-NU126-3ft-Laminar-Flow-Hood/
Magnetic standAmbion

  • Magnetic stand for 96 well plates
  • Column magnets
  • Used for magnetic bead cleanup/size selection steps
L2.10Specs: https://www.thermofisher.com/order/catalog/product/AM10027
Magnetic standDynal
  • Magnetic stand for 96 well plates
  • Bar magnets
  • Used for magnetic bead cleanup/size selection steps
L2.10Specs: https://www.thermofisher.com/order/catalog/product/12027



  • No labels