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Protocols and guides

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Real-time PCR

Application guide

Includes:

  • qPCR basics

  • Experimental design considerations

  • Multiplexing considerations

  • Data analysis

  • Gene expression analysis 

Technical guide

Includes: 

  • Primer and probe design

  • qPCR optimization

  • Instrumentation

  • Troubleshooting

Analysis Software download links:

QX200 Digital Droplet PCR

Application guide 

Includes: 

  • ddPCR basics

  • Experimental design considerations

  • Absolute quantification

  • Copy number variation analysis

  • Rare event detection

  • Data analysis

  • Troubleshooting

Protocol for Evagreen Supermix 

Includes:

  • DNA input requirements

  • Restriction digestion recommendations

  • Reaction setup

  • Thermal cycling conditions

Protocol for Probe Supermix 

Includes:

  • DNA input requirements

  • Restriction digestion recommendations

  • Reaction setup

  • Thermal cycling conditions

Analysis software download link: 

PCR primer design

Nanostring 

Wet lab protocols

nCounter XT Assay Protocol 

Includes assay description and protocols for:

  • nCounter XT Codeset gene expression assay
  • nCounter XT Plex2  expression assay
  • nCounter Vantage RNA assay
  • nCounter Vantage RNA:Protein assay
  • nCounter XT DNA assay
  • nCounter XT gene expression assays for single cells and low input material
  • nCounter Vantage Fusion assay

nCounter Elements XT Reagents 

Includes assay description and protocols for:

  • nCounter Elements XT gene expression assay
  • nCounter Elements XT fusion gene assay
  • nCounter Elements XT DNA assay

nCounter miRNA Expression Assay Protocol 

Includes assay description and protocol for:

  • miRNA expression assay

Data analysis guides

Nanostring nSolver Resource Guide 

Includes links to:

nSolver Software (download here) and User Guides

  • nSolver User Manual
  • nSolver Quick Start Guide
  • Gene Expression Data Analysis Guidelines
    • Data QC
    • Background correction
    • Normalization
    • Assessing differential expression and fold changes
  • CNV Data Analysis Guidelines
  • Plexset Guide
  • Protein Assay Normalization and Data Analysis Guide

nSolver Advanced Analysis Module (download here)

Includes links to:

  • Advanced analysis module user manual
  • Quick Start Guide
  • SNV Analysis Guide
  • Fusion Analysis Guide

nSolver Tutorial Videos

Next Generation Sequencing

Illumina Library preparation protocols

DNA sequencing

RNA sequencing

10X Genomics Chromium Single Cell RNA-seq

How do SPRI beads work?

Tapestation

Protocols

Data Analysis

Short Tandem Repeat (STR) Cell Line Authentication

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