WRH Genomics Facility Equipment

Instruments below can be booked via the WRH PPMS website.

Instrument	Vendor	Applications/Description	Location	Instrument details
Rhapsody HT	BD	Single cell or single nuclei multiomics Assays available: Whole transcriptome analysis Targeted gene expression analysis TCR/BCR Profiling (VDJ) Assay CITE-seq cell surface and intracellular protein profiling ATAC-seq epigenomic profiling	J2.06	https://www.bdbiosciences.com/en-au/products/instruments/single-cell-multiomics-systems/bd-rhapsody-ht-xpress
Digital Droplet PCR System (QX200)	Biorad	Droplet reader (left) Droplet generator (right) Absolute quantification of nucleic acid targets Copies/ul output Rare event detection No standards required Process up to 96 samples per run	L2.09 and L2.11	Specs: http://www.bio-rad.com/en-au/product/qx200-droplet-digital-pcr-system
Nanostring nCounter System	Nanostring	Expression profiling of up to 800 targets within 48 hours Quantify mRNA, miRNA and protein expression No cDNA synthesis necessary Off-the-shelf and custom panels available Process 12 samples per run	L2.09	Specs: https://www.nanostring.com/application/files/2814/8970/9851/LBL-10168-01_PDS_nCounter_System.pdf
Tapestation 4200	Agilent	Quantitatively assess DNA and RNA quality RNA and DNA Integrity Numbers (RIN and DIN) are provided Calculate nucleic acid concentrations and product size Process up to 96 samples per run	L2.09	Specs: http://www.agilent.com/cs/library/datasheets/public/5991-6029EN.pdf
Thermomixer C	Eppendorf	Blocks available for 1.5ml tubes (up to 24) and plates Orbital mixing up to 3,000rpm Interval mixing Temperature range: 1-100 degrees C Timer: 5sec - 99hrs Heated lid available to prevent condensation inside tube lid	L2.10

WIMR Genomics Equipment

Instruments below can be booked using the WIMR PPMS website.

Instrument	Vendor	Applications/Description	Location (room)	Instrument details
CFX96 qPCR	Biorad	Real time PCR 96 well block Gradient function Detection channels:	L2.09	Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf
CFX384 qPCR	Biorad	Real time PCR 384 well block Gradient function Detection channels as above	L2.09	Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf
Miniamp Thermocycler	Thermo Fisher	PCR amplification 96 well block; semi-skirted or non-skirted plates	L2.09	Specs: https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/miniamp-thermal-cyclers-specification-sheet.pdf
Veriti Thermocycler	Applied Biosystems	PCR amplification 96 well block; semi-skirted or non-skirted plates	L2.09	Specs: https://www.thermofisher.com/au/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html
Thermomixer C	Eppendorf	Blocks available for 1.5ml tubes (up to 24) and plates Orbital mixing up to 3,000rpm Interval mixing Temperature range: 1-100 degrees C Timer: 5sec - 99hrs Heated lid available to prevent condensation inside tube lid	L2.10	Specs: https://www.eppendorf.com/au-en/eShop-Products/Temperature-Control-and-Mixing/Instruments/Eppendorf-ThermoMixerC-p-PF-19703
iSeq100	Illumina	Short read sequencing Generates up to 4M reads and 1.2Gbp per run Maximum read length: 150bp PE	L2.09	Specs: https://sapac.illumina.com/systems/sequencing-platforms/iseq/specifications.html