Table of Contents

maxLevel	2

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen	2.5x rSDen
405	50					1
		V450_50B	Pacific Blue, Cascade Blue, AF405, V450, BV421, DAPI, eFluor450, SparkViolet423, SuperBright436	450/50	NA		23.5	58.75
		V510_50A	CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO,eFluor506, SparkViolet538	510/50	502LP		22	55
488	20					2
		SSC	SSC	488/10	NA		NA	NA
		B530_30E	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL	530/30	502LP		22.1	55.25
		B585_42D	PE,PI,DsRed,DiA	585/42	556LP		19.8	49.5
		B670_LPB	PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700	670LP	655LP		21.7	54.25
		B780_60A	PE-Cy7, PE-Vio770	780/60	735LP		21.8	54.8
633	20					3
		R660_20C	APC, AF647, CF647, DiD, DRAQ5, eFluor660	660/20	NA		21.7	54.25
		R780_60A	APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770	780/60	735LP		21.8	54.5

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen	2.5x rSDen
405	50					1
		V450_50B	Pacific Blue, Cascade Blue, AF405, V450, BV421, DAPI, eFluor450, SparkViolet423, SuperBright436	450/50	NA		16	40
		V510_50A	CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538	510/50	502LP		16.1	40.25
488	20					2
		SSC	SSC	488/10	NA		N/A
		B530_30E	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL	530/30	502LP		21.3	53.25
		B585_42D	PE,PI,DsRed,DiA	585/42	556LP		22.9	57.25
		B670_LPB	PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700	670LP	655LP		22.2	55.5
		B780_60A	PE-Cy7, PE-Vio770	780/60	735LP		21.1	52.75
633	20					3
		R660_20C	APC, AF647, CF647, DiD, DRAQ5, eFluor660	660/20	NA		16.1	40.25
		R780_60A	APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770	780/60	735LP		15.6	39

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen	2.5x rSDen
488	20					1
		SSC	SSC	488/10	NA		N/A
		Alexa Fluor 488	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL	530/30	502LP		22.8	57
		PE	PE,PI,DsRed,DiA	585/42	556LP		22.8	57
		PerCP	PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700	670LP	655LP		22.8	57
		PE-Cy7	PE-Cy7, PE-Vio770	780/60	735LP		20.5	51.25
633	20					2
		APC	APC, AF647, CF647, DiD, DRAQ5, eFluor660	660/20	NA		22.8	57
		APC-Cy7	APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770	780/60	735LP		23.3	58.25

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen	2.5x rSDen
488	50					1
		SSC	SSC	488/10	NA		N/A	N/A
		B530_30B	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL	530/30	505LP		24.7	61.75
		B710_50A	PerCP-Cy5.5, 7AAD, BB700, PerCP-eFluor710	710/50	685LP		24.5	61.25
~~405~~	50					2
		~~V440_40F~~	Pacific Blue, Cascade Blue, AF405, V450, BV421, Dylight405, DAPI, eFluor450, SparkViolet423, SuperBrigth436	~~440/40~~	NA		~~20.1~~	~~50.25~~
		~~V525_50E~~	~~CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538~~	~~525/50~~	~~505LP~~		~~23.3~~	~~58.25~~
		~~V610_20D~~	~~Qdot 605, BV605, SuperBright600~~	~~610/20~~	~~600LP~~		~~35.1~~	~~87.75~~
		~~V660_20C~~	~~Qdot 655, BV650, SuperBright645~~	~~660/20~~	~~630LP~~		~~22.6~~	~~56.5~~
		~~V710_50B~~	~~Qdot 700, BV711, SuperBright702~~	~~710/50~~	~~685LP~~		~~28.4~~	71
		~~V780_60A~~	~~BV785, SuperBright780~~	~~780/60~~	~~750LP~~		~~22.6~~	~~56.5~~
355	20					3
		U379_28C	BUV395	379/28	NA		17.9	44.75
		U515_30B	BUV496, DAPI, Hoechst Blue	515/30	450LP		16.5	41.25
		U740_35A	BUV737, Hoechst Red	740/35	690LP		19.2	48
640	40					4
		R670_14C	APC, AF647, CF647, DiD, DRAQ5, SYTOX RED, eFluor660, SparkNIR685	670/14	NA		14.2	35.5
		R730_45B	APC-R700, AF700, DRAQ5, FVS700	730/45	690LP		13.7	34.25
		R780_60A	APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770	780/60	750LP		14.7	36.75
561	100					5
		Y586_15D	PE, DsRed, dTomato, mTangerine, mStrawberry, SparkYG581	586/15	570LP		17.7	44.25
		Y610_20C	PE-Texas Red, mCherry, PE-CF594, AF568, PI, PE-Dazzle, 7AAD, AF594, PE-eFluor610, SparkYG593	610/20	600LP		16.4	41
		Y710_50B	PE-Cy5.5, 7AAD, PE-Fire700	710/50	685LP		16.4	41
		Y780_60A	PE-Cy7, PE-Vio770,	780/60	750LP		19.1	47.75

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen	2.5x rSDen
488	50-100 default @ 50					1
		SSC	SSC	488/10	N/A		N/A	N/A
		B530_30B	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, BODIPY FL, CF488A, DyLight488, EGFP, EYFP, Kiravia Blue520, NovaFluor Blue510, NovaFluor Blue530, SparkBlue550, Sytox Green, VioBright515, VioBright FITC	530/30	505LP		15.1	37.75
		B710_50A	PerCP-Cy5.5, 7AAD, BB700, PerCP-eFluor710, PE-Fire700, PerCP-Vio700	710/50	685LP		15.1	37.75
403	50					2
		V440_40F	Pacific Blue, Cascade Blue, AF405, V450, BV421, Dylight405, DAPI, eFluor450, SparkViolet423, SuperBright436, CellTrace Violet, CF405S, StarBright V440, Sytox Blue, VioBlue	440/40	N/A		10.1	25.25
		V525_50E	Pacific Green, CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538, Krome Orange, StarBright V515, T-Sapphire, VioGreen, VioletFluor500	525/50	505LP		10.1	25.25
		V610_20D	Qdot 605, BV605, SuperBright600, StarBright V610	610/20	600LP		10.5	26.25
		V660_20C	Qdot 655, BV650, SuperBright645, StarBright V670	660/20	630LP		11.4	28.5
		V710_50B	Qdot 700, BV711, SuperBright702, StarBright V710	710/50	685LP		10.5	26.25
		V780_60A	BV785, SuperBright780, StarBright V760, StarBright V790	780/60	750LP		10	25
355	20					3
		U379_28C	BUV395, StarBright UV400	379/28	N/A		20.5	51.25
		U515_30B	BUV496, DAPI, Hoechst Blue, StarBright UV510	515/30	450LP		22.1	55.25
		U740_35A	BUV737, Hoechst Red, StarBright UV740	740_35	690LP		20.4	51
639	40					4
		R670_14C	APC, AF647, CF647, DiD, DRAQ5, eFluor660, SparkNIR685, Celltrace Far Red, DyLight633, DyLight649, NovaFluor Red660, NovaFluor Red685, Sytox ORed	670/14	N/A		15.7	39.25
		R730_45B	APC-R700, AF680, AF700, DRAQ5, FVS700, NovaFluor Red700, R718	730/45	690LP		16.4	41
		R780_60A	APC-Cy7, APC-H7, DiR, APC-Fire750, APC-eFluor780, APCVio770, AF750, APC-AF750	780/60	750LP		17	42.5
561	50					5
		Y586_15D	PE, DsRed, dTomato, mTangerine, mOrange, mStrawberry,SparkYG581, CellTrace Yellow, DyLight549, eFluor570, NovaFluor Yellow570, RY586, Sytox Orange	586/15	570LP		13.6	34
		Y610_20C	PE-Texas Red, mCherry, PE-CF594, AF568, AF594, PI, PE-Dazzle594, PE eFluor610, SparkYG593, BODPIY 581-591-C11, eFluor615, NovaFluor Yellow610, PE-Vio615, StarBright Y605	610/20	600LP		14.9	37.25
		Y710_50B	PE-Cy5.5, 7AAD, PE-Fire700, NovaFluor Yellow690, NovaFluor Yellow700, StarBright Y720	710/50	685LP		13.6	34
		Y780_60A	PE-Cy7, PE-Vio770,	780/60	750LP		15.5	38.75

...

Baseline in use is #23533.
Default voltages are normally good for resolving dim populations. Rule of thumb guide to settings voltages include, ensuring the rSD of the unlabelled cells is at least 2.5x the value of the rSD of electronic noise of the detector (that is viewable from the instrument configuration sheet) that accompanies each cytometer. You need to record a small file in order to accurately determine the rSD of the unlabelled cells each time. Remember this should be the minimum voltage and not the voltage to use. CST voltages are a good voltage to use with the exception that if a population is off scale, then the voltage needs to be reduced.
Filter changes on the Fortessa and LSRII can be done once users have been trained. The “Non-default signage” must be displayed when using a non-default filter in place of the default.
For optimal detection of PE-CY5, PE-AF647 & mPlum a 670/30 filter is needed in Y710_50B with a 635LP.
For optimal detection of PerCP a 675/50 could be used in B710_50A with a 635LP. There are other alternatives too.
For optimal detection of Hoechst Blue & AF350 a 450/50 bandpass filter could be used in U515_30B, with a 425LP.
For optimal detection of AF633 a 670/40 bandpass filter could be used in R670_14C.

Symphony

Baseline: 18 Oct 202305 Feb 2024

Baseline report:

Cytometer Baseline Reportsymphony.pdf

...

Laser Colour	Laser (nm)	Max power (mw)	CST default power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic mirror	Notch filter	Laser order	Pinhole position	Detector specifics	Orientation	Additional notes
Red	637	140	140						1	1		5D
				R820_60	APC-Cy7, APC-H7, DiR, APC-Vio770, APC-Fire810	820/60 BP	800LP				H14121-50
				R780_60	AF750, APC-AF750, APC-eFluor780, APC-Fire750, APC-H7	780/60 BP	750 LP	785nm			H14121-50
				R730_45	APC-R700, AF700, DRAQ5, FVS700, AF680, NovaFluor Red685, NovaFluor Red700, R718	730/45 BP	685 LP				H11900-20
				R670_30	APC, AF647, CF647, DiD, DRAQ5, eFluor660, Spark NIR685, CellTrace Far Red, DyLight633, DyLight649, NovaFluor Red660, Sytox Red	670/30 BP	665 LP				H11900-20		Schott RG665
UV	355	100	100						2	3		M1&M2&M1
				U820_60	BUV805	820/60 BP	800 LP	785nm			H14121-50
				U780_60	spectral	780/60 BP	770 LP				H14121-50
				U740_35	BUV737, Hoechst Red, StarBright UV740	740/35 BP	710 LP				H14121-50
				U695_40		695/40	685 LP
				U670_30	BUV661, StarBright UV665	670/30 BP	635 LP				H11900-20
				U610_20	BUV615, StarBright UV610, StarBright UV605	610/20 BP	600 LP				H11900-20
				U586_15	BUV563	586/15 BP	570 LP	594nm			H11900-20
				U515_30	BUV496, DAPI, Hoechst Blue, StarBright UV510	515/30 BP	450 LP				H11900-20
				U450_50	AF350, DAPI, CellTrace Blue, StarBright UV445	450/50 BP	410 LP				H11900-20
				U379_28	BUV395, StarBright UV400	379/28 BP					H11900-110
Yellow	561	150	150						3	4		6D
				Y820_60	PE-Cy7, StarBright Y800	820/60 BP	800 LP				H14121-50
				Y780_60		780/60 BP	750 LP	785nm			H11900-20
				Y710_50	PE-Cy5.5, PE-Fire700, NovaFluor Yellow690, NovaFluor Yellow700, StarBright Y720	710/50 BP	685 LP				H11900-20
				Y670_30	PE-Cy5, NovaFluor Yellow660, StarBright Y665	670/30 BP	635 LP				H11900-20
				Y610_20	PE-CF594, PE-Texas Red, mCherry, AF568, AF594, PI, PE-Dazzle594, PE eFluor610, SparkYG593, NovaFluor Yellow610, PE-Vio615, StarBright Y605	610/20 BP	600 LP				H11900-20
				Y586_15	PE, DsRed, dTomato, mTangerine, mOrange, mStrawberry, Spark YG581, CellTrace Yellow, DyLight549, eFluor570, NovaFluor Yellow570, R586, Sytox Orange	586/15 BP		594nm Y-58 Schott			H11900-40
Blue	488	200	150						4	5		M1&M2
				B820_60	BB790, StarBright Blue810	820/60 BP	800 LP				H14121-50
				B780_60	RB780	780/60 BP	750 LP	785nm			H14121-50
				B750_30	BB755, StarBright Blue 765	750/30 BP	735 LP				H14121-50
				B710_50	BB700, PerCP-Cy5.5, 7AAD, PerCP-eFluor710, PerCP-Vio700, PE-Fire700, StarBright Blue700	710/50 BP	685 LP				H11900-20
				B670_30	BB660, PerCP, NovaFluor Blue660-40S, NovaFluor Blue610-120S, StarBright Blue675	670/30 BP	635 LP				H11900-20
				B610_20	BB630, NovaFluor Blue610-30S, NovaFluor Blue610-70S, StarBright Blue615	610/20 BP	600 LP	594nm			H11900-20
				B515_30	FITC, BB515, A488, BODIPY FL, CF488A, CSFE, DyLight488, EGFP, Kiravia Blue510, NF-Blue510, NF-Blue530, Spark Blue550, Sytox Green, VioBright515, VioBright FITC	515/30 BP	500 LP				H11900-2040
				SSC		488/10 BP					H11900-20
Orange	594	100	100						5	6		5D
				O660_20	spectral	660/20 BP	635 LP				H14121-20
				O616_23	AF594, mCherry	616/23 BP	610 LP				H14121-20
Violet	406	200	200						6	7		M1&M2&M1
				V820_60	BV786, BV875, SuperBright780, StarBright V790	820/60 BP	800 LP				H14121-50
				V780_60	spectral	780/60 BP	770 LP	785nm			H14121-50
				V750_30	BV750, StarBright V760	750/30 BP	735 LP				H14121-50
				V710_50	BV711, Qdot700, SuperBright702, StarBright V710	710/50 BP	685 LP				H11900-20
				V677_20	BV650, Qdot650, SuperBright645, StarBright V670	677/20 BP	635 LP				H11900-20
				V610_20	BV605, Qdot605, SuperBright600, StarBright V610	610/20 BP	600 LP				H11900-20
				V586_15	BV570, Pacific Orange	586/15 BP	550 LP	594nm			H11900-20
				V525_50	BV510, Pacific Green, CFP, AF430, AmCyan, Cascade Yellow, V500, PO, eFluor506, Spark Violet538, StarBright V515, T-Sapphire, VioGreen, VioletFluor500	525/50 BP	505 LP				H11900-20
				V474_25	BV480, DAPI, StarBright V475	474/25 BP	450 LP				H11900-20
				V427_25	BV421, Pacific Blue, Cascade Blue, AF405, V450, eFluor450, VioBlue, Spark Violet423, StarBright V440, SuperBright436, Sytox Blue	427/25 BP	410 LP				H11900-20
				V405_10	Violet SSC	405/10 BP					H11900-20
						*PMT installed					H11900-20
						*PMT installed					H11900-20
						*PMT installed					H11900-20
IndigoNIR	446_779	75_100	75_100						7	9		5D
				N820_60	DyLight800 (strep-avidin - recommended), AF790	820/60 BP	800 LP				H14121-50
				I660_20	spectral	660/20 BP	635 LP				H14121-20
				I605_40	spectral	605/40 BP	600 LP				H11900-20
				I515_30	spectral	515/30 BP	500 LP				H11900-20
				I470_15	CFP, Chromomycin	470/15 BP	450 LP				H11900-20

...

Baseline reports:

CS&T bead lot: 2004 200

Laser Colour	Laser (nm)	Detector	Band pass filter	Wavelength Start (nm)	Wavelength End (nm)	Laser order
Ultraviolet	355					1
		UV1	373/15	365	380
		UV2	388/15	380	395
		UV3	428/15	420	435
		UV4	443/15	436	451
		UV5	458/15	451	466
		UV6	473/15	466	481
		UV7	514/28	500	528
		UV8	542/28	528	566
		UV9	582/31	566	597
		UV10	613/31	597	628
		UV11	664/27	651	678
		UV12	692/28	678	706
		UV13	720/29	706	735
		UV14	750/30	735	765
		UV15	780/30	765	795
		UV16	812/34	795	829
Violet	405					2
		V1	428/15	420	435
		V2	443/15	436	451
		V3	458/15	451	466
		V4	473/15	466	481
		V5	508/20	498	518
		V6	525/17	516	533
		V7	542/17	533	550
		V8	581/19	571	590
		V9	598/20	588	608
		V10	615/20	605	625
		V11	664/27	651	678
		V12	692/28	678	706
		V13	720/29	706	735
		V14	750/30	735	765
		V15	780/30	765	795
		V16	812/34	795	829
Blue	488					3
		B1	508/20	498	518
		B2	525/17	516	533
		B3	542/17	533	550
		B4	581/19	571	590
		B5	589/20	588	608
		B6	615/20	605	625
		B7	661/17	654	670
		B8	679/18	670	688
		B9	697/19	688	707
		B10	717/20	707	727
		B11	738/31	728	749
		B12	760/23	749	772
		B13	783/23	772	795
		B14	812/34	795	829
Yellow-Green	561					4
		YG1	577/20	567	587
		YG2	598/20	588	608
		YG3	615/20	605	625
		YG4	661/17	653	670
		YG5	679/18	670	688
		YG6	697/19	688	707
		YG7	720/29	706	735
		YG8	750/30	735	765
		YG9	780/30	765	795
		YG10	812/34	795	829
Red	640					5
		R1	661/17	653	670
		R2	679/18	670	688
		R3	697/19	688	707
		R4	717/20	707	727
		R5	738/21	728	749
		R6	760/23	749	772
		R7	783/23	772	795
		R8	812/34	795	829

ARIAIII - Cell Sorter

Baseline: 14 Jan 202210 Nov 2023

Baseline reports:

70umCytometer Baseline Report.pdf

...

130umCytometer Baseline Report.pdf

CS&T bead lot: 3566823533

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order	rSDen (70um)	2.5x rSDen (70um)
633	18					1
		R660_20C	APC, AF647, CF647, DiD, DRAQ5, eFluor660	660/20	NA		7.8	19.5
		R730_45B	APC-R700, AF700, DRAQ5	730/45	690LP		6.7	16.75
		R780_60A	APC-Cy7, APC-H7, DiR, APC-eFL780, APC-Fire750	780/60	750LP		6.1	15.25
488	20					2
		SSC		488/10	NA		N/A
		B530_30E	FITC, GFP, AF488, BBYFP, 5-FAM, CFSE , BB515, ZsGreen, BODIPY FL	530/30	502LP		7.1	17.75
		B585_42D	PE, DsRed, dTomato (sub-optimal), mTangerine (sub-optimal), mStrawberry (sub-optimal)	585/42	556LP		7	17.5
		B616_23C	PE-Texas Red, mCherry (sub-optimal), PE-CF594, PE-AF610, AF568, PE-eFL610, PE-Dazzle594, PE eFluor610	616/23	610LP		7	17.5
		B695_40B	PerCP, PerCP-Cy5.5, 7AAD, PI, PerCP-eFL710, PerCP-Vio700, BB700, PerCP-eFluor710	695/40	655LP		7.6	19
		B780_60A	PE-Cy7, PE-Vio770	780/60	735LP		7	17.5
405	50					3
		V440_40F	Pacific Blue, Cascade Blue, AF405, V450, DAPI, BV421, DyLight 405, eFL450, VioBlue, DAPI, eFluor450, SuperBright436	440/40	NA		6.2	15.5
		V525_50E	CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506	525/50	505LP		5.1	12.75
		V610_20D	BV605, eFL605, Qdot 605, SuperBright600	610/20	600LP		5	12.5
		V660_20C	BV650, eFL650, Qdot 655	660/20	630LP		5.1	12.75
		V710_50B	BV711, Qdot 700, SuperBright702	710/50	685LP		6	15
		V780_60A	BV785, Qdot 800, SuperBright780	780/60	750LP		7.1	17.75

...

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order
488	50-200 default 100					1
		PMT 1 - FSC (Par)	Forward Scatter - Parrallel	488/10	NA
		PMT 2 - FSC (Perp)	Forward Scatter - Perpendicular	488/10	NA
		PMT 3 - SSC	Side Scatter	488/10	NA
		PMT 4	FITC, GFP, AF488, BBYFP, 5-FAM, CFSE , BB515, ZsGreen, DiO, BODIPY FL	530/30	600LP
		PMT 5	PerCP, PerCP-Cy5.5, 7AAD, PI, PerCP-eFL710, PerCP-Vio700, BB700, PerCP-eFluor710	710/50	NA
405	100	405 nm - 100mw				2
		PMT 14	Pacific Blue, Cascade Blue, AF405, V450, BV421, DyLight 405, eFL450, VioBlue, DAPI, eFluor450	440/40	455LP, 500LP
		PMT 15	CFP, eCFP, eFluor506	480/40	500LP
		PMT 16	CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, VioGreen	525/30	550LP
		PMT 17	BV570, Qdot 565,	572/28	585LP, 645LP
		PMT 18	BV605, DyLight405, Qdot 605, SuperBright600	610/20	645LP
		PMT 19	BV650, eFL650, Qdot 655, SuperBright645	660/20	685LP
		PMT 20	BV711, Qdot 700, SuperBright702	710/50	NA
561	150	561 nm - 150mw				3
		PMT 6	PE, DsRed, dTomato, mTangerine, mStrawberry, Dil	585/29	600LP
		PMT 7	PE-Texas Red, mCherry, PE-CF594, PE-AF610, AF568, PE-eFL610, PE-Dazzle594, PE eFluor610	610/20	645LP
		PMT 8	PE-Cy5	670/30	685LP
		PMT 9	PE-Cy5.5	710/50	740LP
		PMT 10	PE-Cy7, PE-Vio770	750LP	NA
355	100	355 nm - 100mw				4
		PMT 13	DAPI, Hoechst Blue	460/50	480LP, 600LP
		PMT 22	BUV395	379/34	427LP
		PMT 23	BUV496	520/35	550LP, 600LP
		PMT 24	BUV661, Hoechst Red	670/30	NA
		PMT_Unplugged	BUV563	585/42	NA
628	120	640 nm - 120mw				5
		PMT 11	APC, AF647, CF647, DiD, DRAQ5,eFluor660	670/30	700LP
		PMT 12	AF700, DRAQ5, APC-R700	740/40	740LP
		PMT 21	APC-Cy7, APC-H7, DiR, APC-eFL780, APC-Fire750	750LP	NA

BD FASDiscover S8 Imaging cell sorter

...

Laser
Detector
Colour Laser (nm) Max power (mw) Detector Common fluorochromes detectable (not exhaustive)
ImagingBlueB534_15FITC, GFP, AF488, BBYFP, 5-FAM, CFSE , BB515, ZsGreen, DiO, BODIPY FLYesB586_42PE, PIYesB700_54PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700
Yes
B783_56PE-Cy7, PE-Vio770YesRedR660_10APC, AF647NoR783_56APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770NoVioletV448_48Pacific Blue, Cascade Blue, AF405, V450, BV421, DAPI, eFluor450, SparkViolet423, SuperBright436NoV528_45BV510, V500, AmCyanNoV605_36BV605NoV755_LPBV786No
Band pass filter Laser order
Imaging 488 40 FSC
N/A

SSC (Imaging)
488/15

Light Loss (Imaging)
488/5

ImgB1
534/46

ImgB2
600/60

ImgB3
788/225

Ultraviolet 355 60

1

UV1
375/20

UV2
392/14

UV3
420/20

UV4
440/20

UV5
459/18

UV6
476/18

UV7
501/16

UV8
517/16

UV9
532/16

UV10
547/16

UV11
574/16

UV12
590/16

UV13
607/18

UV14
625/18

UV15
655/20

UV16
675/20

UV17
698/26

UV18
724/26

UV19
752/30

UV20
782/30

UV21
812/30

UV22
844/24
Violet 405 100

2

SSC (Violet)
405/15

Lightloss (Violet)
405/5

V1
420/20

V2
440/20

V3
459/18

V4
476/16

V5
501/16

V6
517/16

V7
532/16

V8
547/16

V9
574/16

V10
590/16

V11
607/18

V12
625/18

V13
655/20

V14
775/20

V15
698/26

V16
724/26

V17
752/30

V18
782/30

V19
812/30

V20
844/34
Blue 488 40

3

B1
501/16

B2
517/16

B3
532/16

B4
547/16

B5
574/16

B6
590/16

B7
607/18

B8
625/18

B9
655/20

B10
675/20

B11
698/26

B12
724/30

B13
752/30

B14
782/30

B15
812/30

B16
844/34
Yellow-Green 561 100

4

YG1
574/16

YG2
590/16

YG3
607/18

YG4
625/18

YG5
655/20

YG6
675/20

YG7
698/26

YG8
724/26

YG9
752/30

YG10
782/30

YG11
812/30

YG12
844/34
Red 640 100

5

R1
655/20

R2
675/20

R3
698/26

R4
724/26

R5
752/30

R6
782/30

R7
812/30

R8
844/34

Cytoflex - WIMR
Baseline: NA
QC bead lot #: 12APA
Laser (nm)
Power (mw) Detector Common fluorochromes detectable (not exhaustive) Band pass filter Dichroic Mirror Laser order
405
50

2

V405_10
vSSC
405/10
NA

V450_50
DAPI, BV421, Pacific Blue, Cascade Blue, AF405, V450, DyLight 405, eFL450, VioBlue
450/45
NA

488
20

1

SSC
SSC
488/8
NA

B525_40
FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green
525/40
NA

B585_42
PE,PI,DsRed,DiA
585/42
NA

633
20
NOT ACTIVE NOT ACTIVE

3
...

Band pass filter	Laser order
Imaging	488	40	FSC	N/A
			SSC (Imaging)	488/15
			Light Loss (Imaging)	488/5
			ImgB1	534/46
			ImgB2	600/60
			ImgB3	788/225

Ultraviolet	355	60			1
			UV1	375/20
			UV2	392/14
			UV3	420/20
			UV4	440/20
			UV5	459/18
			UV6	476/18
			UV7	501/16
			UV8	517/16
			UV9	532/16
			UV10	547/16
			UV11	574/16
			UV12	590/16
			UV13	607/18
			UV14	625/18
			UV15	655/20
			UV16	675/20
			UV17	698/26
			UV18	724/26
			UV19	752/30
			UV20	782/30
			UV21	812/30
			UV22	844/24
Violet	405	100			2
			SSC (Violet)	405/15
			Lightloss (Violet)	405/5
			V1	420/20
			V2	440/20
			V3	459/18
			V4	476/16
			V5	501/16
			V6	517/16
			V7	532/16
			V8	547/16
			V9	574/16
			V10	590/16
			V11	607/18
			V12	625/18
			V13	655/20
			V14	775/20
			V15	698/26
			V16	724/26
			V17	752/30
			V18	782/30
			V19	812/30
			V20	844/34
Blue	488	40			3
			B1	501/16
			B2	517/16
			B3	532/16
			B4	547/16
			B5	574/16
			B6	590/16
			B7	607/18
			B8	625/18
			B9	655/20
			B10	675/20
			B11	698/26
			B12	724/30
			B13	752/30
			B14	782/30
			B15	812/30
			B16	844/34
Yellow-Green	561	100			4
			YG1	574/16
			YG2	590/16
			YG3	607/18
			YG4	625/18
			YG5	655/20
			YG6	675/20
			YG7	698/26
			YG8	724/26
			YG9	752/30
			YG10	782/30
			YG11	812/30
			YG12	844/34
Red	640	100			5
			R1	655/20
			R2	675/20
			R3	698/26
			R4	724/26
			R5	752/30
			R6	782/30
			R7	812/30
			R8	844/34

Laser (nm)	Power (mw)	Detector	Common fluorochromes detectable (not exhaustive)	Band pass filter	Dichroic Mirror	Laser order
405	50					2
		V405_10	vSSC	405/10	NA
		V450_50	DAPI, BV421, Pacific Blue, Cascade Blue, AF405, V450, DyLight 405, eFL450, VioBlue	450/45	NA
488	20					1
		SSC	SSC	488/8	NA
		B525_40	FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green	525/40	NA
		B585_42	PE,PI,DsRed,DiA	585/42	NA
633	20	NOT ACTIVE	NOT ACTIVE			3

Page Comparison

Versions Compared

Old Version 140

New Version Current

Key

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640

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639

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Symphony

ARIAIII - Cell Sorter

633

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488

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BD FASDiscover S8 Imaging cell sorter

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633

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