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*All data collected will be synced to the WIMR server. If you would like to copy your data elsewhere copy it from this location. Your export folder can be easily accessed from the Scientific Platforms folder under My computer. Your data should be able to be accessed from the Scientific Platforms networked drive within WIMR, and via a username/password for non-WIMR users if desired (email wrhflow@sydneywestmead.cytometry@sydney.edu.au to to setup).
*Data should follow and be stored in the below listed format/location.
- Log in to PPMS screen locker.
- CytExpert should already be open (if it isn't start the software).
- Click new experiment from template.
- Navigate to the template folder and choose a template.
- Make a new experiment in the CytExpert Data Folder using the provided syntax.
- Initialise the instrument by clicking 'Initialise' if needed (if needed the button will be activated).
- Provide 50uL of sample to be counted (with or without facility provided DAPI - 5uL to be added is used). Samples can be provided in eppendorf tubes or 5mL FACS tubes. Templates are set to record 30 seconds of sample @30uL/min. this means 15uL of sample is used. These may be changed to suit your experiment.
- Click 'Run' to start acquisition. Adjust the detector sensitivities if needed using the hand tool.
- Click 'Record' to record 15uL of sample (alternatively the 'Run' data is saved and can be used also).
- Adjust the gate to exclude debri. New gates can be drawn if needed.
- Extract the events per uL statistic from the template.
- If desired export the PDF - final workflow to be determined.
- Save your experiment.
- Log out of PPMS.
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