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- Log in to PPMS screen locker.
- CytExpert should already be open (if it isn't start the software).
- Click new experiment from template.
- Navigate to the template folder and choose a template.
- Make a new experiment in the CytExpert Data Folder using the provided syntax.
- Initialise the instrument by clicking 'Initialise' if needed (if needed the button will be activated).
- Provide 50uL of sample to be counted (with or without facility provided DAPI - 5uL to be added is used). Samples can be provided in eppendorf tubes or 5mL FACS tubes. Templates are set to record 30 40 seconds of sample @30uL/min. this means 15uL 20uL of sample is will be used. These may be changed to suit your experiment.
- Click 'Run' to start acquisition. Adjust the detector sensitivities if needed using the hand tool.
- Click 'Record' to record 15uL 20uL of sample (alternatively the 'Run' data is saved and can be used also).
- Adjust the gate to exclude debri. New gates can be drawn if needed.
- Extract the events per uL statistic from the template.
- If desired export the PDF - final workflow to be determined.
- Save your experiment.
- Log out of PPMS.
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