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This document describes the safe operation of the CytoFLEX flow cytometer located at the Westmead Research Hub Flow Cytometry Core Facility (WRHFlow) in the Westmead Institute for Medical Research (WIMR). This document includes starting up the system, basic operation, cleaning, and shutting down the instruments when necessary by both staff and researchers. The procedures apply to users operating the CytoFLEX in room J.2.06, level 2 of WIMR. All personnel require training prior to independent operation of the instruments. All instrument operation is to be conducted by trained operators. Any assay to be run on the analysers involving hazardous chemicals must have appropriate approval.
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- Acquisition of samples on the CytoFLEX must only occur after the approval of an associated project in PPMS.
- 100mL of 12.5% sodium hypochlorite (undiluted from the provided containers) must be added to the emptied waste containers daily and upon renewing the waste container.
- We recommend filtering samples prior to acquisition on the flow cytometry analysers to CytoFLEX to prevent sample acquisition issues arising from SIP clogging.
- Fixed samples may be filtered immediately before acquisition using the WRHFlow Westmead Cytometry provided 50um filters and plastic tweezers or tube top filters.
- Unfixed samples may be filtered before acquisition using the WRHFlow the Westmead Cytometry provided 50um filters and plastic tweezers or tube top filters in a biological safety cabinet.
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- Under no circumstances should there be any patient/person identiable data recorded on the instruments as the data is not privately held and is accessible to all researchers.
- User data is deleted after 7 days off the instrument computers, it is the responsibility of the user to ensure data has been transferred to a secure location.
- If you would like your data accessible from outside of WIMR, a user specific share can be setup. This works by creating a copy of a specified folder on university provisioned storage that is shared with the user. This requires an email to wrhflow@sydney to Westmead.Cytometry@sydney.edu.au and it is the users responsibility that all ethics approvals are complied with.
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