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Argo SIM QC slide (Manufactured by Argolight France. Purchased in Dec 2021). This slide is composed of different fluorescent patterns in 2D and 3D. Below is an overview of available fluorescent patterns and corresponding quality tests.
Quality Test | Corresponding Pattern | Patten Image | QC Frequency |
Lateral resolution | Gradually spaced lines |  | Monthly |
Intensity response | Intensity gradation (4x4 & 2x16) | Monthly | |
Field uniformity | Field of rings | Monthly | |
Field distortion | Field of rings | Monthly | |
Stage drift during Z-stacking | 3D crossing stairs | Yearly | |
Stage drift during timelapse | Repositioning cross | Yearly | |
Optical sectioning strength | Matrix of crosses | Yearly | |
Accuracy of 3D reconstruction | Sphere | Yearly | |
Line spread function | Field of rings | Yearly | |
Ring spread function | Field of rings |
| Yearly |
Method:
Step1: Image acquisition
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