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Comment: EV - updated ARIA baseline reports

Table of Contents
maxLevel2

...

Laser (nm)

Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser orderrSDen2.5x rSDen

405

50





1



V450_50B

Pacific Blue, Cascade Blue, AF405, V450, BV421, DAPI, eFluor450, SparkViolet423, SuperBright436

450/50

NA


23.5

58.75



V510_50A

CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO,eFluor506, SparkViolet538

510/50

502LP


22

55

488 

20





2



SSC

SSC

488/10

NA


NA

 NA



B530_30E

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL

530/30

502LP


22.1

55.25



B585_42D

PE,PI,DsRed,DiA

585/42

556LP


19.8

49.5



B670_LPB

PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700

670LP

655LP


21.7

54.25



B780_60A

PE-Cy7, PE-Vio770

780/60

735LP


21.8

54.8

633

20





3



R660_20C

APC, AF647, CF647, DiD, DRAQ5, eFluor660

660/20

NA


21.7

54.25



R780_60A

APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770

780/60

735LP


21.8

54.5

...

Laser (nm)Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser orderrSDen2.5x rSDen

405

50





1



V450_50B

Pacific Blue, Cascade Blue, AF405, V450, BV421, DAPI, eFluor450, SparkViolet423, SuperBright436

450/50

NA


1640



V510_50A

CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538

510/50

502LP


16.140.25

488 

20





2



SSC

SSC

488/10

NA


N/A


B530_30E

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL

530/30

502LP

 

21.353.25


B585_42D

PE,PI,DsRed,DiA

585/42

556LP


22.957.25


B670_LPB

PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700

670LP

655LP


22.255.5


B780_60APE-Cy7, PE-Vio770

780/60

735LP


21.152.75

633

20





3



R660_20C

APC, AF647, CF647, DiD, DRAQ5, eFluor660

660/20

NA


16.140.25


R780_60A

APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770

780/60

735LP


15.639

...

Laser (nm)Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser orderrSDen2.5x rSDen

488 

20





1



SSC

SSC

488/10

NA


N/A


Alexa Fluor 488

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL

530/30

502LP

 

22.857


PE

PE,PI,DsRed,DiA

585/42

556LP


22.857


PerCP

PerCP,PI, PerCP-Cy5.5,PerCP-Cy5,PE-Cy5,7AAD, BB700

670LP

655LP


22.857


PE-Cy7PE-Cy7, PE-Vio770

780/60

735LP


20.551.25

633

20





2



APC

APC, AF647, CF647, DiD, DRAQ5, eFluor660

660/20

NA


22.857


APC-Cy7

APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770

780/60

735LP


23.358.25

...

Laser (nm)Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser orderrSDen2.5x rSDen

488

50





1



SSC

SSC

488/10

NA


N/AN/A



B530_30B

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green, BODIPY FL

530/30

505LP


24.761.75


B710_50APerCP-Cy5.5, 7AAD, BB700, PerCP-eFluor710710/50685LP
24.561.25

405

50





2



V440_40FPacific Blue, Cascade Blue, AF405, V450, BV421, Dylight405, DAPI, eFluor450, SparkViolet423, SuperBrigth436440/40NA
20.150.25


V525_50E

CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538

525/50

505LP

 

23.358.25


V610_20D

Qdot 605, BV605, SuperBright600

610/20

600LP


35.187.75


V660_20C

Qdot 655, BV650, SuperBright645

660/20

630LP


22.656.5


V710_50B

Qdot 700, BV711, SuperBright702

710/50

685LP


28.471


V780_60ABV785, SuperBright780780/60750LP
22.656.5

355

20





3



U379_28CBUV395379/28NA
17.944.75


U515_30BBUV496, DAPI, Hoechst Blue515/30450LP
16.541.25


U740_35ABUV737, Hoechst Red740/35690LP
19.248

640

40





4



R670_14CAPC, AF647, CF647, DiD, DRAQ5, SYTOX RED, eFluor660, SparkNIR685670/14NA
14.235.5


R730_45B

APC-R700, AF700, DRAQ5, FVS700

730/45

690LP


13.734.25


R780_60A

APC-Cy7, APC-H7, DiR, APC-FIRE, APC-eFluor780, APCVio770

780/60

750LP


14.736.75

561

100





5



Y586_15DPE, DsRed, dTomato, mTangerine, mStrawberry, SparkYG581586/15570LP
17.744.25


Y610_20C

PE-Texas Red, mCherry, PE-CF594, AF568, PI, PE-Dazzle, 7AAD, AF594, PE-eFluor610, SparkYG593

610/20600LP
16.441


Y710_50BPE-Cy5.5, 7AAD, PE-Fire700710/50685LP
16.441


Y780_60APE-Cy7, PE-Vio770, 780/60750LP
19.147.75

...

Laser (nm)Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser orderrSDen2.5x rSDen

488

50-100

default @ 50





1



SSC

SSC

488/10

N/A


N/AN/A



B530_30B

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, BODIPY FL, CF488A, DyLight488, EGFP, EYFP, Kiravia Blue520, NovaFluor Blue510, NovaFluor Blue530, SparkBlue550, Sytox Green, VioBright515, VioBright FITC

530/30

505LP


15.137.75


B710_50APerCP-Cy5.5, 7AAD, BB700, PerCP-eFluor710, PE-Fire700, PerCP-Vio700710/50685LP
15.137.75

403

50





2



V440_40FPacific Blue, Cascade Blue, AF405, V450, BV421, Dylight405, DAPI, eFluor450, SparkViolet423, SuperBright436, CellTrace Violet, CF405S, StarBright V440, Sytox Blue, VioBlue440/40N/A
10.125.25


V525_50E

Pacific Green, CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, eFluor506, SparkViolet538, Krome Orange, StarBright V515, T-Sapphire, VioGreen, VioletFluor500

525/50

505LP

 

10.125.25


V610_20D

Qdot 605, BV605, SuperBright600, StarBright V610

610/20

600LP


10.526.25


V660_20C

Qdot 655, BV650, SuperBright645, StarBright V670

660/20

630LP


11.428.5


V710_50B

Qdot 700, BV711, SuperBright702, StarBright V710

710/50

685LP


10.526.25


V780_60ABV785, SuperBright780, StarBright V760, StarBright V790780/60750LP
1025

355

20





3



U379_28CBUV395, StarBright UV400379/28N/A
20.551.25


U515_30BBUV496, DAPI, Hoechst Blue, StarBright UV510515/30450LP
22.155.25


U740_35ABUV737, Hoechst Red, StarBright UV740740_35690LP
20.451

639

40





4



R670_14CAPC, AF647, CF647, DiD, DRAQ5, eFluor660, SparkNIR685, Celltrace Far Red, DyLight633, DyLight649, NovaFluor Red660, NovaFluor Red685, Sytox ORed670/14N/A
15.739.25


R730_45B

APC-R700, AF680, AF700, DRAQ5, FVS700, NovaFluor Red700, R718

730/45

690LP


16.441


R780_60A

APC-Cy7, APC-H7, DiR, APC-Fire750, APC-eFluor780, APCVio770, AF750, APC-AF750

780/60

750LP


1742.5

561

50





5



Y586_15DPE, DsRed, dTomato, mTangerine, mOrange, mStrawberry,SparkYG581, CellTrace Yellow, DyLight549, eFluor570, NovaFluor Yellow570, RY586, Sytox Orange586/15570LP
13.634


Y610_20C

PE-Texas Red, mCherry, PE-CF594, AF568, AF594, PI, PE-Dazzle594, PE eFluor610, SparkYG593, BODPIY 581-591-C11, eFluor615, NovaFluor Yellow610, PE-Vio615, StarBright Y605

610/20600LP
14.937.25


Y710_50BPE-Cy5.5, 7AAD, PE-Fire700, NovaFluor Yellow690, NovaFluor Yellow700, StarBright Y720710/50685LP
13.634


Y780_60APE-Cy7, PE-Vio770, 780/60750LP
15.538.75

...

Laser ColourLaser (nm)Max power (mw)CST default power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic mirrorNotch filterLaser orderPinhole positionDetector specificsOrientationAdditional notes
Red637140140




11
5D




R820_60APC-Cy7, APC-H7, DiR, APC-Vio770, APC-Fire810820/60 BP800LP


H14121-50





R780_60AF750, APC-AF750, APC-eFluor780, APC-Fire750, APC-H7780/60 BP750 LP785nm

H14121-50





R730_45APC-R700, AF700, DRAQ5, FVS700, AF680, NovaFluor Red685, NovaFluor Red700, R718730/45 BP685 LP


H11900-20





R670_30APC, AF647, CF647, DiD, DRAQ5, eFluor660, Spark NIR685, CellTrace Far Red, DyLight633, DyLight649, NovaFluor Red660, Sytox Red670/30 BP665 LP


H11900-20
Schott RG665
UV355100100




23
M1&M2&M1




U820_60BUV805820/60 BP800 LP785nm

H14121-50





U780_60spectral780/60 BP770 LP


H14121-50





U740_35BUV737, Hoechst Red, StarBright UV740740/35 BP710 LP


H14121-50





U695_40
695/40685 LP









U670_30BUV661, StarBright UV665670/30 BP635 LP


H11900-20





U610_20BUV615, StarBright UV610, StarBright UV605610/20 BP600 LP


H11900-20





U586_15BUV563586/15 BP570 LP594nm

H11900-20





U515_30BUV496, DAPI, Hoechst Blue, StarBright UV510515/30 BP450 LP


H11900-20





U450_50AF350, DAPI, CellTrace Blue, StarBright UV445450/50 BP410 LP


H11900-20





U379_28BUV395, StarBright UV400379/28 BP



H11900-110

Yellow561150150




34
6D




Y820_60PE-Cy7, StarBright Y800820/60 BP800 LP


H14121-50





Y780_60
780/60 BP750 LP785nm

H11900-20





Y710_50PE-Cy5.5, PE-Fire700, NovaFluor Yellow690, NovaFluor Yellow700, StarBright Y720710/50 BP685 LP


H11900-20





Y670_30PE-Cy5, NovaFluor Yellow660, StarBright Y665670/30 BP635 LP


H11900-20





Y610_20PE-CF594, PE-Texas Red, mCherry, AF568, AF594, PI, PE-Dazzle594, PE eFluor610, SparkYG593, NovaFluor Yellow610, PE-Vio615, StarBright Y605610/20 BP600 LP


H11900-20





Y586_15PE, DsRed, dTomato, mTangerine, mOrange, mStrawberry, Spark YG581, CellTrace Yellow, DyLight549, eFluor570, NovaFluor Yellow570, R586, Sytox Orange586/15 BP

594nm

Y-58 Schott



H11900-40

Blue488200150




45
M1&M2




B820_60BB790, StarBright Blue810820/60 BP800 LP


H14121-50





B780_60RB780780/60 BP750 LP785nm

H14121-50





B750_30BB755, StarBright Blue 765750/30 BP735 LP


H14121-50





B710_50BB700, PerCP-Cy5.5, 7AAD, PerCP-eFluor710, PerCP-Vio700, PE-Fire700, StarBright Blue700710/50 BP685 LP


H11900-20





B670_30BB660, PerCP, NovaFluor Blue660-40S, NovaFluor Blue610-120S, StarBright Blue675670/30 BP635 LP


H11900-20





B610_20BB630, NovaFluor Blue610-30S, NovaFluor Blue610-70S, StarBright Blue615610/20 BP600 LP594nm

H11900-20





B515_30FITC, BB515, A488, BODIPY FL, CF488A, CSFE, DyLight488, EGFP, Kiravia Blue510, NF-Blue510, NF-Blue530, Spark Blue550, Sytox Green, VioBright515, VioBright FITC515/30 BP500 LP


H11900-20





SSC
488/10 BP



H11900-20

Orange594100100




56
5D




O660_20spectral660/20 BP635 LP


H14121-20





O616_23AF594, mCherry616/23 BP610 LP


H14121-20

Violet406200200




67
M1&M2&M1




V820_60BV786, BV875, SuperBright780, StarBright V790820/60 BP800 LP


H14121-50





V780_60spectral780/60 BP770 LP785nm 

H14121-50





V750_30BV750, StarBright V760750/30 BP735 LP


H14121-50





V710_50BV711, Qdot700, SuperBright702, StarBright V710710/50 BP685 LP


H11900-20





V677_20BV650, Qdot650, SuperBright645, StarBright V670677/20 BP635 LP


H11900-20





V610_20BV605, Qdot605, SuperBright600, StarBright V610610/20 BP600 LP


H11900-20





V586_15BV570, Pacific Orange586/15 BP550 LP594nm

H11900-20





V525_50BV510, Pacific Green, CFP, AF430, AmCyan, Cascade Yellow, V500, PO, eFluor506, Spark Violet538, StarBright V515, T-Sapphire, VioGreen, VioletFluor500525/50 BP505 LP


H11900-20





V474_25BV480, DAPI, StarBright V475474/25 BP450 LP


H11900-20





V427_25BV421, Pacific Blue, Cascade Blue, AF405, V450, eFluor450, VioBlue,  Spark Violet423, StarBright V440, SuperBright436, Sytox Blue427/25 BP410 LP


H11900-20





V405_10Violet SSC405/10 BP



H11900-20







*PMT installed



H11900-20







*PMT installed



H11900-20







*PMT installed



H11900-20

IndigoNIR446_77975_10075_100




79
5D




N820_60DyLight800 (strep-avidin - recommended), AF790820/60 BP800 LP


H14121-50





I660_20spectral660/20 BP635 LP


H14121-20





I605_40spectral605/40 BP600 LP


H11900-20





I515_30spectral515/30 BP500 LP


H11900-20





I470_15CFP, Chromomycin470/15 BP450 LP


H11900-20

...

ARIAIII - Cell Sorter

Baseline: 14 Jan  

Baseline reports:

70umCytometer Baseline Report.pdf

...

Laser (nm)

Power

(mw)

DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser order

488

50-200

default 100





1



PMT 1 - FSC (Par)Forward Scatter - Parrallel488/10NA



PMT 2 - FSC (Perp)Forward Scatter - Perpendicular488/10NA


PMT 3 - SSCSide Scatter488/10NA


PMT 4FITC, GFP, AF488, BBYFP, 5-FAM, CFSE , BB515, ZsGreen, DiO, BODIPY FL530/30600LP


PMT 5PerCP, PerCP-Cy5.5, 7AAD, PI, PerCP-eFL710, PerCP-Vio700, BB700, PerCP-eFluor710710/50NA
405100405 nm - 100mw   2


PMT 14Pacific Blue, Cascade Blue, AF405, V450, BV421, DyLight 405, eFL450, VioBlue, DAPI, eFluor450440/40455LP, 500LP


PMT 15CFP, eCFP, eFluor506480/40500LP


PMT 16CFP, AF430, AmCyan, Cascade Yellow, V500, BV510, PO, VioGreen525/30550LP


PMT 17BV570, Qdot 565, 572/28585LP, 645LP


PMT 18BV605, DyLight405, Qdot 605, SuperBright600610/20645LP


PMT 19BV650, eFL650, Qdot 655, SuperBright645660/20685LP


PMT 20BV711, Qdot 700, SuperBright702710/50NA
561150561 nm - 150mw   3


PMT 6PE, DsRed, dTomato, mTangerine, mStrawberry, Dil585/29600LP


PMT 7PE-Texas Red, mCherry, PE-CF594, PE-AF610, AF568, PE-eFL610, PE-Dazzle594,  PE eFluor610610/20645LP


PMT 8PE-Cy5670/30685LP


PMT 9PE-Cy5.5710/50740LP


PMT 10PE-Cy7, PE-Vio770750LPNA
355100355 nm - 100mw   4


PMT 13DAPI, Hoechst Blue460/50480LP, 600LP


PMT 22BUV395379/34427LP


PMT 23BUV496520/35550LP, 600LP


PMT 24BUV661, Hoechst Red670/30NA


PMT_UnpluggedBUV563585/42NA
628120640 nm - 120mw   5


PMT 11APC, AF647, CF647, DiD, DRAQ5,eFluor660670/30700LP


PMT 12AF700, DRAQ5, APC-R700740/40740LP


PMT 21APC-Cy7, APC-H7, DiR, APC-eFL780, APC-Fire750750LPNA

...

Laser (nm)

Power (mw)DetectorCommon fluorochromes detectable (not exhaustive)Band pass filterDichroic MirrorLaser order

405

50





2


V405_10

vSSC

405/10

NA




V450_50

DAPI, BV421, Pacific Blue, Cascade Blue, AF405, V450, DyLight 405, eFL450, VioBlue

450/45

NA


488 

20





1


SSC

SSC

488/8

NA




B525_40

FITC, GFP, AF488, YFP, 5-FAM, CFSE, BB515, ZsGreen, SYBR Green

525/40

NA




B585_42

PE,PI,DsRed,DiA

585/42

NA


633

20

NOT ACTIVENOT ACTIVE

3

...