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Quality Test | Corresponding Pattern | Patten Image | QC Frequency |
Lateral resolution | Gradually spaced lines | Monthly | |
Intensity response | Intensity gradation (4x4 & 2x16) | Monthly | |
Field uniformity | Field of rings | Monthly | |
Field distortion | Field of rings | Monthly | |
Stage drift during Z-stacking | 3D crossing stairs | Yearly | |
Stage drift during timelapse | Repositioning cross | Yearly | |
Optical sectioning strength | Matrix of crosses | Yearly | |
Accuracy of 3D reconstruction | Sphere | Yearly | |
Line spread function | Field of rings | Yearly | |
Ring spread function | Field of rings |
| Yearly |
Method:
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