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Date of approval: 7/7/2020
Approval authority: Scientific Platforms Manager, Workplace Health and Safety Manager, Advanced Specialist Flow Cytometry
Functional unit: Flow Cytometry
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Monday to Friday
Advanced Specialist on 0-8627 3216
Cytometry/Imaging/EM Manager on 0-8627 1820
Scientific Platforms Manager platforms Director on 0-8627 3210
Email: westmead.cytometry@sydney.edu.au
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All new users require instrument training with a relevant training record completed. Initial access is not limited to business hours. Initial usage of the instrument is to be with a trained user.
Instrument booking guidelines
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Task or scenario | Hazard/s | Associated harm | Existing risk controls | Current risk rating | Additional risk controls? | Residual risk rating? |
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| Instrument operation | Electrocution | Contact with electricity can cause electric shock and burns | Routine instrument maintenance - to ensure instrument is in good condition and cabling is not damaged. Electrical equipment annual testing. Educate users to prevent liquid spill into the instrument. Safety circuit breakers and fuses on instrument to prevent general electrocution due to instrument failure, especially in the presence of liquid. Emergency power off button located in laboratories to disconnects power to the red power points and not the blue uninterruptible power points. | low | ||
| Mechanical hazard | Moving parts can cause injury to body parts | Educate users to keep the Trough, Sleeves, and Safety Shield attached when operating. When the instrument’s rotors are rotating, do not touch them or load or remove tubes from the instrument. Always stop or abort a procedure before any adjustments. Warning signs on instrument moving parts. | low | |||
Exposure to bio-hazardous material | Exposure to biohazardous material can cause health issues. | PPE – gloves, gown & enclosed shoes. Biological spill kit - Access to emergency biological spill kit and/or cleaning equipment. Ethanol decontamination of instrument performed during shutdown. Project approval process. | low | |||
Hot surface hazard | The heating foil of the gentleMACS heaters may become hot enough to cause burns if touched. | Educate users not to touch the heating foil, while the instrument is in operation. Warning signs on heating parts | low | |||
Sample handling | Contact with bio-hazardous material | Exposure to bio-hazardous material | Handle samples in biosafety cabinet PPE – gloves, gown & enclosed shoes are necessary for working in the laboratory. Access to emergency biological spill kit and materials to clean up spills. | low |
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In these procedures the following terms have the meaning set out below
- C tubes- Purple-cap C Tubes to get viable single-cell suspensions for cell separation, culture, or analysis.
- M tubes - Orange-cap M Tubes to achieve thorough sample homogenization for subsequent molecular and microbiology analysis.
List of Resources Required
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- In case of spillage or contamination, clean the instrument appropriately.
- For decontamination, wipe the instrument with 70% ethanol. Clean the instrument only with cleaning agents that are non-corrosive and not damaging to the plastic
housing of the gentleMACS Octo Dissociator with Heaters. Suitable cleaning agents include household detergents and 70% ethanol. - Power off the instrument
Emergency procedures
Emergency procedure folder can be found here in the sample prep lab:
You can also access all these information via WIMR intranet:
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