WRH Genomics Facility Equipment

Instruments below can be booked via the WRH PPMS website.

Instrument	Vendor	Applications/Description	Location	Instrument details
C1000 Thermocycler	Biorad	PCR amplification 96 well block Gradient function	L2.09	Specs: http://www.bio-rad.com/en-au/product/thermal-cyclers-for-pcr/c1000-touch-thermal-cycler
Covaris Evolution Ultrasonicator	Covaris	Precise DNA and RNA shearing to specified fragment lengths Chromatin shearing for ChIP samples DNA and RNA extraction from FFPE samples Process up to 8 samples per run	J2.06	Specs: http://covarisinc.com/products/afa-ultrasonication/e220evolution/
Digital Droplet PCR System (QX200)	Biorad	Droplet reader (left) Droplet generator (right) Absolute quantification of nucleic acid targets Copies/ul output Rare event detection No standards required Process up to 96 samples per run	L2.09 and L2.11	Specs: http://www.bio-rad.com/en-au/product/qx200-droplet-digital-pcr-system
Nanostring nCounter System	Nanostring	Expression profiling of up to 800 targets within 48 hours Quantify mRNA, miRNA and protein expression No cDNA synthesis necessary Off-the-shelf and custom panels available Process 12 samples per run	L2.09	Specs: https://www.nanostring.com/application/files/2814/8970/9851/LBL-10168-01_PDS_nCounter_System.pdf
Tapestation 4200	Agilent	Quantitatively assess DNA and RNA quality RNA and DNA Integrity Numbers (RIN and DIN) are provided Calculate nucleic acid concentrations and product size Process up to 96 samples per run	L2.09	Specs: http://www.agilent.com/cs/library/datasheets/public/5991-6029EN.pdf
Thermomixer C	Eppendorf	Blocks available for 1.5ml tubes (up to 24) and plates Orbital mixing up to 3,000rpm Interval mixing Temperature range: 1-100 degrees C Timer: 5sec - 99hrs Heated lid available to prevent condensation inside tube lid	L2.10

WIMR Genomics Equipment

Instruments below can be booked using the WIMR PPMS website.

Instrument	Vendor	Applications/Description	Location	Instrument details
CFX96 qPCR	Biorad	Real time PCR 96 well block Gradient function Detection channels:	L2.09	Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf
CFX384 qPCR	Biorad	Real time PCR 384 well block Gradient function Detection channels as above	L2.09	Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf
Stratagene MX3005P	Integrated Sciences	Real time PCR 96 well block; semi-skirted or unskirted plates only Detection channels: FAM/Sybr, HEX/JOE/VIC, Alexa Fluor 350, Tet, Tamra, Cy3, ROX/Texas Red, Cy5	L2.09	Specs: https://www.agilent.com/en/mx3005p-qpcr-systems-details-specifications
Veriti Thermocycler	Applied Biosystems	PCR amplification 96 well block; semi-skirted or non skirted plates	L2.09	Specs: https://www.thermofisher.com/au/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html
Pre-PCR hood	Nuaire	Laminar airflow workstation HEPA filtered Contains UV light for decontamination	L2.11	Specs: https://www.biocompare.com/19657-Laminar-Flow-Hoods-Laminar-Flow-Cabinets/73710-NU126-3ft-Laminar-Flow-Hood/
Magnetic stand	Ambion	Magnetic stand for 96 well plates Column magnets Used for magnetic bead cleanup/size selection steps	L2.10	Specs: https://www.thermofisher.com/order/catalog/product/AM10027
Magnetic stand	Dynal	Magnetic stand for 96 well plates Bar magnets Used for magnetic bead cleanup/size selection steps	L2.09 and L2.10	Specs: https://www.thermofisher.com/order/catalog/product/12331D#/12331D (L2.09) Specs: https://www.thermofisher.com/order/catalog/product/12027 (L2.10)