WRHFlow provides access to the following instruments via PPMS.

BD FASDiscover S8 Imaging cell sorter

Configuration

5 laser digital cytometer with 62 fluorescence detectors

Imaging capability on 3 blue laser parameters

Violet SSC

6 simultaneous sort options

96, 384 well plate capable

Index sorting

CYTEK Aurora

configuration

5 laser digital full spectrum flow cytometer with 64 fluorescent detectors and spectral un-mixing

High throughput plate acquisition is to be installed in the upcoming months

BD Symphony - SORP

configuration

8 laser digital flow cytometer with 50 parameter detection with spectral un-mixing

High throughput 96/384 well plate acquisition capable

Options include APE Quantiflash, cascade arrays, coherent laser control, violet SSC.

BD Fortessa - SORP

configuration

5 laser digital flow cytometer with 20 parameter detection

High throughput 96/384 well plate acquisition capable

BD LSRII

configuration

5 laser digital flow cytometer with 20 parameter detection

High throughput 96/384 well plate acquisition capable

BD CANTOII (x2)

configuration

3 laser digital flow cytometer with 10 parameter detection

Automatic tube loader and high throughput 96/384 well plate acquisition capable

Beckman Coulter - CytoFlex

2 laser digital flow cytometer (cell counter) with 4 parameter detection

Violet SSC

BD INFLUX

configuration

5 laser digital flow cytometer with 24 parameter detection & sorting capability

6 simultaneous sort options

96 well / 384 well / aux. sort collection device

Small particle detector

Polarised FSC detectors

Index sorting

BD ARIAIII

configuration

3 laser digital flow cytometer with 16 parameter detection & sorting capability

4 simultaneous sort options

MILTENYI AUTOMACS PRO

Automated magnetic bulk cell separation

MILTENYIGENTLEMACS

Automated & reproducible cell/nucleic acid extraction from tissues

Heated tissue digestion

EMD MILLIPORELUMINEX X200

Multiplex analyte analysis

96 well plate in <45min

Up to 100 analytes per sample (80 on MagPlex microspheres)

ULTIMAKER

EXTENDED 3+ 3D PRINTER

information

20-200um Z resolution,

20-100^oC heat bed,

Print dimensions of 215x215x300mm

Active levelling,

Dual extrusion print heads,

Materials currently available include PLA, PVA, PP, CPE, CPE+

BIOTEK ELX50 PLATE WASHER

Automated 96 well plate aspiration and buffer dispenser

SARTORIOSELECTRONIC 12-CHANNEL PIPETTE

8 or 12 channel usability

Stepping function

Digital display

microUSB charge

BENCHMARK ORBITAL SHAKER

4 plate capacity

Digital timer and shaking speed setting

SEAHORSE XFe24 ANALYSER

SPECTRAMAX ID5 PLATE READER

Absorbance, luminescence, fluorescence, spectral readings, spectral scanning readings, fluorescence lifetime.

End-point, kinetic, well area scanning.

Heater and shaking compatible.

Workstation Precision3680

Local analysis workstation located in C.2.09

Provides access to FlowJo & SpectraFlo

ARGUS ANALYSIS TERMINAL x 3

Provided by Sydney Informatics Hub - see here

Access requires unikey registration via email to westmead.cytometry@sydney.edu.au

Provides access to FlowJo (site licence required), Huygens, Matlab, Cell Profiler, ImageJ, R, RStudio and other flow/imaging software

16 vCPUs @ 2.2GHz/3.2GHz

8GB GPUs

300GB SSD / 1TB

128GB RAM

#1: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, FJ (licence needed)

#2: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, FJ (licence needed), Olyvia,

#3: Huygens, ImageJ(Fiji), histoCAT, cellprofiler, MATLAB, Rstudio, R, Olyvia, cellSens

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