This BYOC was designed as one of my goals of the ISAC SRL EL program to act as an additional aid to spark interest when teaching cytometry to students. It has been designed to be affordable ($500-$1500), 3D printable and fun to build. I have included build list and instructions and highlight there are many opportunities for improvement/modification.
The BYOC Workshop was first held @ ACS 2016 in Sydney, Australia, @ ACS 2018 in Adelaide, Australia and conjointly with the Garvan Flow Facility @ ACS 2019, Melbourne, Australia.
Check back for updates.
Overview
The cytometer uses a <$50 adjustable 5-25mW laser (interchangeable) that is directed onto a jet in air stream. The stream is ejected throughout a 100um nozzle that is assembled using luer locks, cogs and HPLC tubing to allow injection of sample in a laminar flow. The laser is focused onto the stream and signals are detected using a 25mm, 25mm focal lens that directs the light onto the FSC/SSC/FL detectors. A diagram of the light path from excitation is shown in the diagram below. The signals are detected with a APD/Photodiode or PMT and then converted to a voltage output using a pre-amp. The signals can be viewed using an oscilloscope or can be displayed on a bivariate dot plot using a ADC connected to a computer.
3D Printable Parts
Steering laser mirror mount - https://www.thingiverse.com/thing:3523131
Flow cell - https://www.thingiverse.com/thing:3902321
Detector - https://www.thingiverse.com/thing:3902266
Mounting - https://www.thingiverse.com/thing:3902335
Sample tube mount - https://www.thingiverse.com/thing:3902328
Flow control - https://www.thingiverse.com/thing:3902432
Laser mount - https://www.thingiverse.com/thing:3902366
2019 - Acrylic Parts
to be uploaded
Materials - 3D Printed Version (acrylic assists can be interchanged)
| Item | Qty | Purpose | Sources | Cost ($AUD) | Notes | Image |
|---|---|---|---|---|---|---|
| 600x600 tile | 1 | base | Local tile supplier | Free (tell them what you are making) | ||
| 600x600 steel sheet | 1 | build platform | Bunnings (Sydney) | $26 | Ensure it is magnetic. Thickness does not matter, thinner normally means cheaper. I recommend sign steel. Have some fun and wobble it ;p | |
| 450 extension springs | 19 (2 detectors), additional 6 per detector | provides pulling force to mirror, laser, lenses | Bolts & Industrial (Sydney) Century Springs (US) | $4 | We need to buy it from a reseller. Spring Catalogue: https://www.centurysprings.com.au/products/prod_catalogue.htm |
|
| M3 socket screw | bag | |||||
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