Note -

• Scattered signals are not displayed as they are irrelevant. Quantiflash does not emit well <400nm hence BUV395 plot is N/A & in the NIR hence why these plots look odd.
• Recorded .fcs files need to be in a subfolder called 'laser_on'
• Recorded .fcs files need to be in a subfolder called 'laser_off'
• With this data - an optimal range of voltages can be determined. 
• Height parameter used - has in testing matched manual voltration in testing. 

Protocol - 

1. New experiment, all lasers on. 
2. Set laser delays to 0
3. Set all scaling factors to 0.5 (inc. FSC)
4. Acquire data with QF scripter automatically changing the QF intensity every second - 1000 events/second, gaussian pulse - standard settings (FSC set to 400V)
5. Set FSC so that all H/W FSC signals are on scale. 
6. Set events to record to 20k.
7. Record data with PMT volts from 0-1000 (25V increments) - PM for app to do this unattended (vchanger.exe)
8. Put data in sub folder labelled 'laser_on' and 'laser_off' and run R script (updated in 2022)

SIGNAL:NOISE

VOLTAGES - V1 - voltages_2022_04.xlsx

knees.csv signal to noise data

LINEARITY

CV