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WRH Genomics Facility Equipment

Instruments below can be booked via the WRH PPMS website. 

InstrumentVendorImageApplications/DescriptionLocationInstrument details
C1000 Thermocycler


Biorad
               
  • PCR/amplification
  • 96 well block
  • Gradient function
L2.10

Specs: http://www.bio-rad.com/en-au/product/thermal-cyclers-for-pcr/c1000-touch-thermal-cycler

Covaris Evolution UltrasonicatorCovaris

              

  • Precise DNA and RNA shearing to specified fragment lengths
  • Chromatin shearing for ChIP samples
  • DNA and RNA extraction from FFPE samples
  • Process up to 8 samples per run
J2.06

Specs: http://covarisinc.com/products/afa-ultrasonication/e220evolution/

Digital Droplet PCR System (QX200)Biorad

  • Droplet reader (left)
  • Droplet generator (right)
  • Absolute quantification of nucleic acid targets
  • Copies/ul output
  • Rare event detection
  • No standards required
  • Process up to 96 samples per run
L2.09 and L2.10

Specs: http://www.bio-rad.com/en-au/product/qx200-droplet-digital-pcr-system

Nanostring nCounter SystemNanostring

  • Expression profiling of up to 800 targets within 48 hours
  • Quantify mRNA, miRNA and protein expression
  • No cDNA synthesis necessary
  • Off-the-shelf and custom panels available
  • Process 12 samples per run
L2.10Specs: https://www.nanostring.com/application/files/2814/8970/9851/LBL-10168-01_PDS_nCounter_System.pdf
Tapestation 4200Agilent

                         

  • Quantitatively assess DNA and RNA quality 
  • RNA and DNA Integrity Numbers (RIN and DIN) are provided
  • Calculate nucleic acid concentrations and product size
  • Process up to 96 samples per run
L2.10

Specs: http://www.agilent.com/cs/library/datasheets/public/5991-6029EN.pdf

WIMR Genomics Equipment

Instruments below can be booked using the WIMR PPMS website.

InstrumentVendorImageApplications/DescriptionLocationInstrument details
CFX96 qPCR Biorad

     

  • Real time PCR
  • 96 well block
  • Gradient function
  • Detection channels:
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf
CFX384 qPCRBiorad

      

  • Real time PCR
  • 384 well block
  • Gradient function
  • Detection channels as above
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf
Stratagene MX3005PIntegrated Sciences

  • Real time PCR
  • 96 well block; semi-skirted or unskirted plates only
  • Detection channels:
    • FAM/Sybr, HEX/JOE/VIC, Alexa Fluor 350, Tet, Tamra, Cy3, ROX/Texas Red, Cy5
L2.09Specs: https://www.agilent.com/en/mx3005p-qpcr-systems-details-specifications
Rotorgene 3000

Qiagen

        

  • Real time PCR
  • Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
  • Detection channels: 4 colours
    • FAM/Sybr, JOE, ROX, Cy5
L2.09Specs: http://www.lth.se/fileadmin/sciblu/QC/RG3000.pdf
Rotorgene 6000Qiagen

  • Real time PCR
  • Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
  • Detection channels: 5 colours
    • FAM/Sybr, JOE, ROX, Cy3, Cy5, 
L2.09Specs are for Rotorgene Q (upgraded model): https://www.qiagen.com/us/shop/automated-solutions/pcr-instruments/rotor-gene-q/#orderinginformation














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