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WRH Genomics Facility Equipment

Instruments below can be booked via the WRH PPMS website. 

Specs: http
InstrumentVendorImageApplications/DescriptionLocationInstrument details
C1000 ThermocyclerBiorad
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  • PCR amplification
  • 96 well block
  • Gradient function
L2.09
Rhapsody HTBD


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  • Single cell or single nuclei multiomics
  • Assays available:
    • Whole transcriptome analysis
    • Targeted gene expression analysis
    • TCR/BCR Profiling (VDJ) Assay
    • CITE-seq cell surface and intracellular protein profiling
    • ATAC-seq epigenomic profiling
J2.06https://www.
bio-rad
bdbiosciences.com/en-au/
product
products/instruments/
thermal
single-
cyclers
cell-
for
multiomics-
pcr
systems/
c1000
bd-
touch
rhapsody-
thermal-cyclerCovaris Evolution UltrasonicatorCovaris

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  • Precise DNA and RNA shearing to specified fragment lengths
  • Chromatin shearing for ChIP samples
  • DNA and RNA extraction from FFPE samples
  • Process up to 8 samples per run
J2.06

Specs: http://covarisinc.com/products/afa-ultrasonication/e220evolution/

ht-xpress
Digital Droplet PCR System (QX200)Biorad

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  • Droplet reader (left)
  • Droplet generator (right)
  • Absolute quantification of nucleic acid targets
  • Copies/ul output
  • Rare event detection
  • No standards required
  • Process up to 96 samples per run
L2.09 and L2.11

Specs: http://www.bio-rad.com/en-au/product/qx200-droplet-digital-pcr-system

Nanostring nCounter SystemNanostring

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  • Expression profiling of up to 800 targets within 48 hours
  • Quantify mRNA, miRNA and protein expression
  • No cDNA synthesis necessary
  • Off-the-shelf and custom panels available
  • Process 12 samples per run
L2.09Specs: https://www.nanostring.com/application/files/2814/8970/9851/LBL-10168-01_PDS_nCounter_System.pdf
Tapestation 4200Agilent

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  • Quantitatively assess DNA and RNA quality 
  • RNA and DNA Integrity Numbers (RIN and DIN) are provided
  • Calculate nucleic acid concentrations and product size
  • Process up to 96 samples per run
L2.09

Specs: http://www.agilent.com/cs/library/datasheets/public/5991-6029EN.pdf

Thermomixer CEppendorf

                    Eppendorf ThermoMixer_C with SmartBlock_1.5 mLImage Modified

  • Blocks available for 1.5ml tubes (up to 24) and plates
  • Orbital mixing up to 3,000rpm
  • Interval mixing
  • Temperature range: 1-100 degrees C
  • Timer: 5sec - 99hrs
  • Heated lid available to prevent condensation inside tube lid
L2.10


WIMR Genomics Equipment

Instruments below can be booked using the WIMR PPMS website.

Real time PCR unskirted plates onlyDetection channels:FAM/Sybr, HEX/JOE/VIC, Alexa Fluor 350, Tet, Tamra, Cy3, ROX/Texas Red, Cy5wwwagilent/en/mx3005p-qpcr-systems-details-specificationsSpecs: biocompare19657Laminar-Flow-Hoods-Laminar-Flow-Cabinets/73710-NU126-3ft-Laminar-Flow-Hood/
InstrumentVendorImageApplications/DescriptionLocation (room)Instrument details
CFX96 qPCR Biorad

     

  • Real time PCR
  • 96 well block
  • Gradient function
  • Detection channels:
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf
CFX384 qPCRBiorad

      

  • Real time PCR
  • 384 well block
  • Gradient function
  • Detection channels as above
L2.09 Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdfStratagene MX3005PIntegrated Sciences

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Miniamp ThermocyclerThermo FisherImage Added
  • PCR amplification
  • 96 well block; semi-skirted or
  • non-skirted plates
L2.09Specs: https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/miniamp-thermal-cyclers-specification-sheet.pdf
Veriti ThermocyclerApplied Biosystems

   

  • PCR amplification
  • 96 well block; semi-skirted or non-skirted plates
L2.09Specs: https://www.thermofisher.com/au/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.htmlPre-PCR hoodNuaire

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  • Laminar airflow workstation
  • HEPA filtered
  • Contains UV light for decontamination
L2.11
Thermomixer CEppendorfEppendorf ThermoMixer_C with SmartBlock_1.5 mLImage Added
  • Blocks available for 1.5ml tubes (up to 24) and plates
  • Orbital mixing up to 3,000rpm
  • Interval mixing
  • Temperature range: 1-100 degrees C
  • Timer: 5sec - 99hrs
  • Heated lid available to prevent condensation inside tube lid
L2.10Specs: https://www.eppendorf.com/au-
Magnetic standAmbion

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  • Magnetic stand for 96 well plates
  • Column magnets
  • Used for magnetic bead cleanup/size selection steps
L2.10Specs: https://www.thermofisher.com/order/catalog/product/AM10027
Magnetic standDynalImage Removed
  • Magnetic stand for 96 well plates
  • Bar magnets
  • Used for magnetic bead cleanup/size selection steps
L2.09 and L2.10en/eShop-Products/Temperature-Control-and-Mixing/Instruments/Eppendorf-ThermoMixerC-p-PF-19703
iSeq100Illumina

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  • Short read sequencing
  • Generates up to 4M reads and 1.2Gbp per run
  • Maximum read length: 150bp PE
L2.09Specs: https://wwwsapac.thermofisherillumina.com/ordersystems/catalogsequencing-platforms/product/12331D#/12331D (L2.09)Specs: https://www.thermofisher.com/order/catalog/product/12027 (L2.10)iseq/specifications.html