Page Comparison

Real-time PCR

Application guide

Includes:

• qPCR basics

• Experimental design considerations

• Multiplexing considerations

• Data analysis

• Gene expression analysis 

Technical guide

Includes: 

• Primer and probe design

• qPCR optimization

• Instrumentation

• Troubleshooting

Analysis Software download links:

• Biorad CFX Manager

• Rotorgene Q

  • Installation serial number: 050610 

QX200 Digital Droplet PCR

Application guide 

Includes: 

• ddPCR basics

• Experimental design considerations

• Absolute quantification

• Copy number variation analysis

• Rare event detection

• Data analysis

• Troubleshooting

Protocol for Evagreen Supermix 

Includes:

• DNA input requirements

• Restriction digestion recommendations

• Reaction setup

• Thermal cycling conditions

Protocol for Probe Supermix 

Includes:

• DNA input requirements

• Restriction digestion recommendations

• Reaction setup

• Thermal cycling conditions

Analysis software download link: 

• Quantasoft

   

Nanostring 

Wet lab protocols

nCounter XT Assay Protocol 

Includes assay description and protocols for:

• nCounter XT Codeset gene expression assay
• nCounter XT Plex²  expression assay
• nCounter Vantage RNA assay
• nCounter Vantage RNA:Protein assay
• nCounter XT DNA assay
• nCounter XT gene expression assays for single cells and low input material
• nCounter Vantage Fusion assay

nCounter Elements XT Reagents 

Includes assay description and protocols for:

• nCounter Elements XT gene expression assay
• nCounter Elements XT fusion gene assay
• nCounter Elements XT DNA assay

nCounter miRNA Expression Assay Protocol 

Includes assay description and protocol for:

• miRNA expression assay

Data analysis guides

Nanostring nSolver Resource Guide 

Includes links to:

nSolver Software (download here) and User Guides

• nSolver User Manual
• nSolver Quick Start Guide
• Gene Expression Data Analysis Guidelines
  • Data QC
  • Background correction
  • Normalization
  • Assessing differential expression and fold changes
• CNV Data Analysis Guidelines
• Plexset Guide
• Protein Assay Normalization and Data Analysis Guide

nSolver Advanced Analysis Module (download here)

Includes links to:

• Advanced analysis module user manual
• Quick Start Guide
• SNV Analysis Guide
• Fusion Analysis Guide

nSolver Tutorial Videos

• Links to videos are in the nSolver Resource Guide