WRH Genomics Facility Equipment
Instruments below can be booked via the WRH PPMS website.
Specs: http://www.bio-rad| Instrument | Vendor | Image | Applications/Description | Location | Instrument details |
|---|
C1000 ThermocyclerBiorad |  Image Removed | - PCR/amplification
- 96 well block
- Gradient function
| L2.10 | | Rhapsody HT | BD |  Image Added | - Single cell or single nuclei multiomics
- Assays available:
- Whole transcriptome analysis
- Targeted gene expression analysis
- TCR/BCR Profiling (VDJ) Assay
- CITE-seq cell surface and intracellular protein profiling
- ATAC-seq epigenomic profiling
| J2.06 | https://www.bdbiosciences.com/en-au/ |
productthermalcyclersforpcrc1000touchthermal-cycler| Covaris Evolution Ultrasonicator | Covaris |  Image Removed | - Precise DNA and RNA shearing to specified fragment lengths
- Chromatin shearing for ChIP samples
- DNA and RNA extraction from FFPE samples
- Process up to 8 samples per run
| J2.06 | Specs: http://covarisinc.com/products/afa-ultrasonication/e220evolution/ |
| ht-xpress |
| Digital Droplet PCR System (QX200) | Biorad |  Image Modified
| - Droplet reader (left)
- Droplet generator (right)
- Absolute quantification of nucleic acid targets
- Copies/ul output
- Rare event detection
- No standards required
- Process up to 96 samples per run
| L2.09 and L2. |
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WIMR Genomics Equipment
Instruments below can be booked using the WIMR PPMS website.
| Instrument | Vendor | Image | Applications/Description | Location (room) | Instrument details |
|---|
| CFX96 qPCR | Biorad | | - Real time PCR
- 96 well block
- Gradient function
- Detection channels:
 | L2.09 | Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6075.pdf |
| CFX384 qPCR | Biorad | | - Real time PCR
- 384 well block
- Gradient function
- Detection channels as above
| L2.09 | Specs: http://www.biorad.com/webroot/web/pdf/lsr/literature/Bulletin_6072.pdf | Stratagene MX3005P | Integrated Sciences |  Image Removed
| Real time PCR
| Miniamp Thermocycler | Thermo Fisher |  Image Added | - PCR amplification
- 96 well block; semi-skirted or
| unskirted plates onlyDetection channels:FAM/Sybr, HEX/JOE/VIC, Alexa Fluor 350, Tet, Tamra, Cy3, ROX/Texas Red, Cy5 | L2.09 | Specs: https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/miniamp-thermal-cyclers-specification-sheet.pdf |
| Veriti Thermocycler | Applied Biosystems |  Image Added | - PCR amplification
- 96 well block; semi-skirted or non-skirted plates
| L2.09 | Specs: https://www.agilentthermofisher.com/au/en/mx3005p-qpcr-systems-details-specifications |
| Rotorgene 3000 | Qiagen |  Image Removed | - Real time PCR
- Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
- Detection channels: 4 colours
| L2.09 | Specs: http://www.lth.se/fileadmin/sciblu/QC/RG3000.pdf |
| Rotorgene 6000 | Qiagen |  Image Removed
| - Real time PCR
- Rotor format; 0.1ml strip tubes for up to 72 samples, gene disc for 100 samples
- Detection channels: 5 colours
- FAM/Sybr, JOE, ROX, Cy3, Cy5,
| L2.09 | Specs are for Rotorgene Q (upgraded model): https://www.qiagen.com/us/shop/automated-solutions/pcr-instruments/rotor-gene-q/#orderinginformationhome/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html |
| Thermomixer C | Eppendorf |  Image Added | - Blocks available for 1.5ml tubes (up to 24) and plates
- Orbital mixing up to 3,000rpm
- Interval mixing
- Temperature range: 1-100 degrees C
- Timer: 5sec - 99hrs
- Heated lid available to prevent condensation inside tube lid
| L2.10 | Specs: https://www.eppendorf.com/au-en/eShop-Products/Temperature-Control-and-Mixing/Instruments/Eppendorf-ThermoMixerC-p-PF-19703 |
| iSeq100 | Illumina |  Image Added
| - Short read sequencing
- Generates up to 4M reads and 1.2Gbp per run
- Maximum read length: 150bp PE
| L2.09 | Specs: https://sapac.illumina.com/systems/sequencing-platforms/iseq/specifications.html |
