Requesting Training
If you are a PhD student or researcher, please complete a training form on PPMS Table of Contents
Training Request on PPMS
For any training requests to access and use the facility equipment please submit a training request:
https://au.ppms.info/wrh/req/?pf=2&training=true&form=44
Once your request has been submitted, we will arrange a training session for you within 2 weeks.
Training for Honours Student
If you are an honours student, the cytometry laboratory induction & flow cytometry training session should be part of your course work.
You only need to complete the following two forms and submit to westemad.cytometry@sydney.org.au
View file name Record of Laboratory Safety Training and PC2 Laboratory Access Approval - Suat Signed.pdf height 250 View file name WIMR-FM-SP-FC-36.01 Flow Cytometry Training Record_Analysers - Suat Signed Induction&Training.pdf height 250
Flow Analysers Training
The basic analysers training New user trainings are held on the second and fourth Thursdays of the Month. Other types of training will be arranged within 2 weeks of training request submission.
Flow Analyser Training
This training is a charged 2 hours face-to-face session covering:
- Flow cytometry laboratory induction and safety
- Components and how a flow cytometer works
- Workflow to run an experiment focusing on BD FACSDiva software
It will grant you access to:
- BD FACS Canto II
- BD LSR II
- BD LSRFortessa
It will be a 2-3 hours You can see the configurations of the cytometers here:
FACSymphony Training
This is a charged 30 min face-to-face training session covering
Once training is completed, you will need to pass an online competency quiz to gain office hours access:
https://goo.gl/forms/R9UePsEc1Vc3WCrs1
Afterhours access can be granted after 10 hours of experience or, if needed earlier, upon completion of an autonomous access online quiz:
https://forms.gle/BqS7GAAv8PdoyCVQ8
...
To gain access to the FACS SymphonyFACSymphony, you will also need to complete the basic flow analyser training first.
GentleMACS & AutoMACS Training
Cytoflex Training.
Cytek Aurora Training
This is a charged 30 min face-to-face training session if you have already completed the basic flow analyser training.
GentleMACS Training
This is a charged 30 min face-to-face training session.
AutoMACS Training
This is a charged 30 min face-to-face training session.
Cytoflex Training
This is a charged 30 min face-to-face training session.
AriaIII Training
Cell sorting is normally provided as a service. However if you plan to perform cell sorting after hours, you can request to be trained on the AriaIII.
This is a charged 3 x 2 hours face-to-face training session covering:
- AriaIII instrument setup
- Principles of cell sorting
- Experimental setup to sort your target population
- Safety procedures during a sample clog