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Date of revision and approval
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Enquiries contact name: Xin Wang, Suat Dervish, Edwin Lau
Enquiries contact: westmead.cytometry@sydney.edu.au
Objective
This document describes the safe operation of the CytoFLEX flow cytometer located at the Westmead Research Hub Flow Cytometry Core Facility in the Westmead Institute for Medical Research (WIMR). This document includes starting up the system, basic operation, cleaning, and shutting down the instruments when necessary by both staff and researchers. The procedures apply to users operating the CytoFLEX in room J.2.06, level 2 of WIMR. All personnel require training prior to independent operation of the instruments. All instrument operation is to be conducted by trained operators. Any assay to be run on the analysers involving hazardous chemicals must have appropriate approval. OGTR requirements for safe work in a PC2 laboratory apply.
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8.30am to 5.00pm
Monday to Friday
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Friday
Advanced Specialist on 0-8627 3216
Cytometry/Imaging/EM Manager on 0-8627 1820
Scientific Platforms Manager platforms Director on 0-8627 3210
Email: westmead.cytometry@sydney.edu.au
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Training is conducted by a super user or the scientific platforms staff with competency demonstration necessary before authorisation of access. Competency is assessed via completion of a competency quiz.
Emergency procedures
Emergency procedure folder can be found here in the sample prep lab:
You can also access all these information via WIMR intranet:
https://intranet.westmeadinstitute.org.au/workingatwmi/HandS/Pages/Sarah%20Johnston.aspx
All emergencies need to be reported to the emergency contacts listed above. Specific chemical exposure procedures are below.
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