Flow Cytometry User Group Meeting – 8th August 10am – Conference Room C2.22.
We would like to revive the Monthly Cytometry User Group meetings as a forum where users can discuss cytometry related problems, issues, triumphs and successes.
This will be an open forum where users are encouraged to bring data along to share and work through.
The first one will include “Cytometry Tips & Tricks” as well as some discussion in regards to building/sharing core panels to be utilised across labs.
When: August 8th 10am-12pm.
Where: Level 2 Conference Room, WIMR.
What: Cytometry Tips & user discussion.
Did you say colourful? BD Symphony is outputting high quality data.
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Training takes ~30minutes if you already cytometer access and requests are accepted through PPMS.
Spectral Unmixing - Improve your population resolution…
FlowJo 10.6 was released yesterday (after months of beta testing)! This update allowed weighted non-negative least squares fitting for spectral unmixing of your cytometry data!
What does this mean… Instead of compensating your data, you can now spectrally unmix, essentially using all the detectors as reference points for the fluorochromes spectra.
You will need too (and should have been already) ensure all instrument detectors are active and collecting signals as well as ensuring all signals are not off scale for non-primary detectors.
The process is almost similar to compensating using FlowJo but just involves clicking 2 more items in the software when compensating your data.
And you guessed it – this is where the Symphony excels with its numerous detectors.
The shared data analysis computer has had the upgrade but see here for further information.
Additionally this can be performed retrospectivly on standard cytometers if the data was collected with additonal detectors enabled.
Example spectral data - BV737 detected across many detectors.
Fancy Using A Supercomputer? I did (and loved it).
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If you have any concerns please contact us.
Help Out A User 😊
If you can assist with Cindy’s request please contact her via - c.kok@sydney.edu.au
“I’m looking for a CD44 antibody that reacts to either human or rat. Could you please help me to ask around to see if anyone could spare an aliquot of 1-2 uL?”
If you can assist with Jason’s request please contact him via - jher8412@uni.sydney.edu.au“I was hoping you would be able to help me search for a Daudi cell line. My group is interested in investigating NK cell ADCC of cell lines after stimulation with viral antigens. For this we would like to acquire some Daudi cells and if possible we were hoping there may be a group within the Westmead community that may have these cells. Could you please circulate this request through the Westmead community.”