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spython recipe Dockerfile >> Singularity.trinity |
Next,
...
edit
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the
...
newly
...
created
...
...
...
Add
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the
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Artemis-specific
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changes
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to
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your
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new
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singularity
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image,
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i.e.
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Bootstrap: docker From: ubuntu:16.04 %files %Dockerfile $SRC/Dockerfile.$TRINITY_VERSION %labels MAINTAINER bhaas@broadinstitute.org %post mkdir /project /scratch |
Next
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use
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Singularity
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to
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build
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the
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image
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(~30
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minutes
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on
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my
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local
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laptop).
This image/container will be around 2GB as it contains trinity+all the dependencies+the whole operating system. This is fairly standard for Singularity images.
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sudo singularity build Singularity.trinity.build Singularity.trinity |
Now move the build to Artemis and run it with a PBS scriptNow move the build to Artemis (with scp, filezilla, etc) and run it with a PBS script
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#!/bin/bash #PBS -P PROJECT #PBS -l select=1:ncpus=4:mem=4gb #PBS -l walltime=0:10:00 module load singularity/2.6.1 #Change into the directory you executed qsub from cd "$PBS_O_WORKDIR" #Run the singularity image, which contains the program Trinity #The dataset "reads.fq.gz" should be in this directory, #This will create persistent output in a folder called "trinity_out_dir" singularity exec Singularity.trinity.build Trinity --seqType fq --single `pwd`/reads.fq.gz --max_memory 4G --CPU $NCPUS --output `pwd`/trinity_out_dir |
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