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Real-time PCR

Application guide

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Includes:

  • qPCR basics
  • Experimental design considerations
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis 

Technical guide

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Includes: 

  • Primer and probe design
  • qPCR optimization
  • Instrumentation
  • Troubleshooting

QX200 Digital Droplet PCR

Application guide

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Includes: 

  • ddPCR basics
  • Experimental design considerations
  • Absolute quantification
  • Copy number variation analysis
  • Rare event detection
  • Data analysis
  • Troubleshooting

Protocol for Evagreen

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Supermix 

Includes:

  • DNA input requirements
  • Restriction digestion recommendations
  • Reaction setup
  • Thermal cycling conditions

Protocol for Probe

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Supermix 

Includes:

  • DNA input requirements
  • Restriction digestion recommendations
  • Reaction setup
  • Thermal cycling conditions

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