Real-time PCR
Application guide
Includes:
qPCR basics
Experimental design considerations
Multiplexing considerations
Data analysis
Gene expression analysis
Technical guide
Includes:
Primer and probe design
qPCR optimization
Instrumentation
Troubleshooting
Analysis Software download links:
Installation serial number: 050610
QX200 Digital Droplet PCR
Application guide
Includes:
ddPCR basics
Experimental design considerations
Absolute quantification
Copy number variation analysis
Rare event detection
Data analysis
Troubleshooting
Protocol for Evagreen Supermix
Includes:
DNA input requirements
Restriction digestion recommendations
Reaction setup
Thermal cycling conditions
Protocol for Probe Supermix
Includes:
DNA input requirements
Restriction digestion recommendations
Reaction setup
Thermal cycling conditions
Analysis software download link:
Nanostring
Wet lab protocols
nCounter XT Assay Protocol
Includes assay description and protocols for:
- nCounter XT Codeset gene expression assay
- nCounter XT Plex2 expression assay
- nCounter Vantage RNA assay
- nCounter Vantage RNA:Protein assay
- nCounter XT DNA assay
- nCounter XT gene expression assays for single cells and low input material
- nCounter Vantage Fusion assay
nCounter Elements XT Reagents
Includes assay description and protocols for:
- nCounter Elements XT gene expression assay
- nCounter Elements XT fusion gene assay
- nCounter Elements XT DNA assay
nCounter miRNA Expression Assay Protocol
Includes assay description and protocol for:
- miRNA expression assay
Data analysis guides
Nanostring nSolver Resource Guide
Includes links to:
nSolver Software (download here) and User Guides
- nSolver User Manual
- nSolver Quick Start Guide
- Gene Expression Data Analysis Guidelines
- Data QC
- Background correction
- Normalization
- Assessing differential expression and fold changes
- CNV Data Analysis Guidelines
- Plexset Guide
- Protein Assay Normalization and Data Analysis Guide
nSolver Advanced Analysis Module (download here)
Includes links to:
- Advanced analysis module user manual
- Quick Start Guide
- SNV Analysis Guide
- Fusion Analysis Guide
nSolver Tutorial Videos
- Links to videos are in the nSolver Resource Guide