8 Peak Beads – Now available to users
8 peak beads are now available for users to acquire as a means of QC on the WRHFlow cytometers. Users can find the 8 peak beads alongside the QC(CST) beads in the flow cytometry fridge in the shared lab. Acquiring 8 peak beads before and after your acquisition provides information on whether the instrument has changed from the start to the end of your run. They can also be used to set/monitor PMT signals between acquisitions performed on different days in synergy with application setting for those users who are using application users. Please ensure you record the bead lot number!
Instrument Baselines – November 2018
From 2017 to the current, we have maintained the same target MFIs on the 2 CantoIIs and the Fortessa via an almost daily quality control (note: LSRII baseline had been updated earlier in 2018). This has allowed users to utilise application settings for longitudinal studies. The below notice is for users currently using application settings on the instruments.
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New Baseline Implementation Schedule
Instrument | Date Scheduled | Bead Lot |
CantoII_ICPMR | 5/11/2018 | #70466 > #80998 |
CantoII_WIMR | 12/11/2018 | #70466 > #80998 |
LSRII | 31/10/2018 | #70466 > #80998 |
Fortessa | 19/11/2018 | #70466 > #80998 |
Symphony | 17/9/2018 | #70466 > #80998 |
Please remember we recommend compensation controls to be made and acquired in a similar manner and in parallel with your samples. We also recommend running and recording calibration beads both before and after your acquisitions to not only show the instrument was stable throughout the acquisition but to provide target MFIs in the event that application settings cannot be used on subsequent days.
FlowJo Basic and Advanced Tutorial – Save the date!
November 1st 2-5pm – WIMR Seminar Room C2.20.
We will be hosting Jack Panopoulos – FlowJo Application Scientist – as he delivers updates on basic and advanced flow cytometry data analysis using FlowJo & SeqGeq. There have been a number of recently released plugins that there will also be focus on that should interest many.
NanoFCM demonstration – Get your 7-500nm samples ready!
September 19th & - October 15th – WRHFlow Labs - J2.05
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Please note as this is an instrument evaluation user data may be used to furnish a study report of instrument applicability. Also any data will need to be provided to NanoFCM for review prior to publication. If you are interested in the instrument I would suggest evaluating it with a sample of interest, especially while the engineers are here on site. Further instrument information can be obtained here - https://www.nanofcm.com/en/Product/NanoFCM/117.html
BD Symphony – 8 laser, high sensitivity flow cytometer now installed, training for high parameter panel users.
August 22nd - 11am & 2pm - J2.07
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View configuration_V2 for panel design here
CantoII HTS installed – Time to think about preparing samples in 96 well plates?
All our flow cytometers have now been upgraded with high throughput capabilities. That’s a total of 5 high throughput sampling robots in the flow cytometry facility.
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If you would like a 96 well V bottom plate, these can be ordered through PPMS for $6 each.
BD Symphony install - First Symphony A5.2 (not A5.1) install worldwide!
You may notice a number of engineers in the flow labs this week with the BD Symphony installation/testing underway. Geoff Osborne (BD SORP director) and the team will be busy installing the world’s first updated A5.2 which has a revised detector configuration and new electronics.
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Failed laser teardown!
Curious what happens inside a diode laser like the below used on the Fortessa? We were un/lucky enough to have had the red laser fail on the Fortessa in August 2018 and so we did a laser teardown.
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2018 Tips and tricks competition winners announced!
Congratulations to Kirstie Bertram and Nicole Fewings for winning the WRHFlow Tips & Tricks competition.
Kirstie’s entry highlight a number of important points to consider including antibody clone / manufacturer differences when selecting antibodies for a panel, while Nicole’s entry demonstrates the benefits of titrating not only antibodies but viability dyes. See the/wiki/spaces/DMT/pages/400916724for winning entries.
Instrument bookings...
In the last 6 months the 4 flow cytometry analysers located at WRHFlow have seen over 100 different users resulting in over 2000 hours of actual collective usage, with about 1/4 of usage being after business hours. These numbers are wonderful and depict an increasing usage of the instruments.
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