Fortessa HTS upgrade!
The Fortessa now has the hardware required for acquisition from a 96/384 well plate. We have organised an en-masse training session on the 12th of July @ 9am for any one who would like to use it.
Data / Information / Spotify MAC now in LSRII/CantoII lab
Browse the internet for cytometry related information, click through the WRHFlow homepage or listen to tunes in Spotify while acquiring data in J2.07.
Informal flow cytometry data analysis - 26/7/18, 4pm, WIMR conference room C2.20
Like the plugins available in FlowJo, interested in flowCore in R, fancy t-SNE plots...
Bring a notebook, some data, and let's collectively contribute to the data analysis confluence page while hopefully learning a trick or two from peers. This will be an informal get together where users can ask, contribute or add wisdom about anything flow cytometry data analysis related.
Session to be confirmed!
Tube loader & high throughput sampler installations on all cytometers
We are planning the installation of an automated tube loader on the CantoII as well as high throughput samplers on the Fortessa and CantoII over the next 3 weeks. We will be booking ~4 hours on each instrument to complete the installations and are very excited to see these installed. Keep an eye on the latest news page to see the install.
Notice - SIP sheath for the LSRII and Fortessa
The LSRII and Fortessa must have SIP sheath covers installed to prevent dripping of samples.
- The SIP sheath cover is installed by default on both instruments.
- The SIP sheath cover can be removed (but must be reinstalled immediately for a different sample or following your session) for the following samples only if -
- Bead samples are immediately to be acquired, OR,
- The samples have been inactivated by fixation. An example protocol for sample fixation could consist of 20 minutes exposure @ 4°C to a flow cytometry specific commercial fixative containing paraformaldehyde (example buffer set & protocol). Other protocols that result in fixation of samples are also valid, OR
- The samples are being acquired from a HTS device.
- A properly installed SIP sheath cover will look slightly thicker than without the SIP sheath (compare with below photo) and should not drip liquid when the instrument is on run. To remove the SIP sheath on the LSRII or the Fortessa follow these instructions. To replace it, the order is reversed.
- To reinstall the SIP sheath cover follow the reverse instructions. The above applies to the LSRII & Symphony.
A tweaked data export workflow for users
WRHFlow have instigated some automatic data syncing scripts for users who would like to try them out. Unless your data should not be synced to the WIMR server, when exporting your data, export it as FCS3.0 format, with linear format selected to 'D:\BDExport\FCS' (as this folder will from now be automatically synced to the WIMR server). If you would like to copy your data elsewhere you can also copy it from this location. Your data should be able to be accessed from the Scientific Platforms networked drive within WIMR, and via a username/password for non-WIMR users via Dropbox/Onedrive if desired (email wrhflow@sydney.edu.au to setup an account).
Drop off your sample for multiplex analyte analysis?
We are considering implementing a core facility run multiplex assay service to be run on the Luminex 200 to enable users with fewer than 76 samples to merge with other researchers to complete a 96 well kit that is used for multianalyte analysis of chemokines, cytokines, growth factors, etc.
Users would register a spot on a 96 well plate for a particular day. There would be 76 available sample locations as 20 are reserved for standards and QC. On the day, users would drop off their samples, on ice & labelled to the flow cytometry lab by a certain time. WRHFlow would then centrifuge samples, load them, and follow the protocol using automated means followed by plate acquisition on the instrument. The data would then be disseminated to researchers.
We would like to collect feedback that if this was a service provided would it be utilised by you or your group, please let us know via this feedback form over the next 2 weeks.
16th-18th July 2018 - Oz Single Cells 2018
≤September 2018 - New 50 parameter instrument to be installed at WRHFlow
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