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WRH flow users, email your entry with any accompanying image to wrhflow@sydney.edu.au! We have 2 x $100 JB Hifi gift vouchers to give out to the best flow cytometry tips or tricks. Judging will be via a panel of 3 and winning submissions will be posted online to the WRHFlow Tips & Tricks page. The competition will close on the 31st of July 2018.
An example:
Avoid clumps in your sample - filter them through a mesh filter
Flow cytometry is a single cell analysis platform (for the most part). Clumping cells can ruin data acquisition as they may clog the narrow orifice the sample needs to travel through to enter the flow cell resulting in incorrect signals being assigned to events due to the way the instruments works. Filtering your samples before running on the cytometer can assist in minimising this occurring. Other things to try might include adding 2mM EDTA into your buffer, enriching cells from debri/dead cells/free DNA before acquisition, keeping your sample cold, and diluting your sample.
Good practice includes having a time vs. fluorescent parameter (off the last laser used) to monitor your acquisition to spot problems and fix them before finishing running your sample.
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