...

20180612 - LSRII UV laser 

*update - 20180704 - 'B530_30B' PMT replaced. Instrument aligned. All specifications exceeded. New baseline recorded on the 4/7/2018 called WMI_2018_3B_6V_3U_3R_4Y.

It is recommended to note the UV laser change date for your reference.

If continuing application settings see below (otherwise make a new experiment and proceed as normal).

The current suggested workflow involves

1. Seek assistance from WRHFlow staff if not 100% sure of the following procedure (some users may not have access to switch configurations).
2. Switch to the instrument configuration with the baseline recorded on the 29/11/2017 labelled 'WMI_2015_3B_6V_3U_3R_4Y' from the current baseline recorded on the '04/07/2018' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
3. Run performance check 'CST' using bead lot #70466 on 'WMI_2015_3B_6V_3U_3R_4Y' configuration
4. Once completed, there should be an error detected with the UV laser. Click OK and close the CST window. 
5. Create a new experiment.
6. Apply application settings. Note the application settings applied should have been created between 29/11/2017 and '04/07/2018'.
7. Record all voltages using a screen shot. 
8. Switch to the instrument configuration with the baseline recorded on the '04/07/2018' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
9. Create a new experiment, enter the voltages from the screen capture into the cytometer settings.
10. Save these application settings as updated application settings that will now be linked with the baseline recorded on '04/07/2018' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
11. Proceed as normal applying the updated application settings to your experiments.

We strongly recommend that you make note of the UV laser change and the B530_30B PMT change in your experiment records, review the UV parameters and other parameters and record new compensation controls.

*update - 20180629 - R780_60A detector fixed.

...