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*update - 20180628 - The old LSRII UV laser had been exhibiting a declining UV laser power output. This occurs over time as the laser is now was >4 years old. After repeated presentation of performance issues, and a measured output of 9.8 milliwatts 8mW (specification is 2020mW), the engineer replaced the UV laser on the 28th of June 2018. The new UV laser is exhibiting excellent performance (19.8 milliwatt 8mW output and CVs a CV below 3% on the primary detector) but we now need to confront the reality that the baseline has changed and how this affects current application setting users.
Currently 2 other detectors (Blue - B & Red - A) are not performing perfectly (but are still very usable) and hence we have not recorded a new baseline. We will attempt to solve this ASAP. Currently we are continuing with the existing baseline recorded on the 29th of November 2017 because of this. The QC correctly identifies that CST has failed and that the UV detector voltages need to be reduced by ~100 volts (due to the new laser installation). This means that if you are using application settings, the instrument will set your detector voltages ~100 volts lower in order to put the positive peak in the same spot as what it was. This will result in your negative population being lower and/or less spread for the current application setting users. It is recommended that new compensation beads be recorded for any experiment.
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Continuing Application settings (this only refers to users once will apply once the above 2 detectors have been fixed and a new baseline is recorded and users notified recorded with subsequent notification via the information screen that displays after logging in to the analysis PC)...
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- Seek assistance from WRHFlow staff if not 100% sure of the following procedure.
- Switch to the instrument configuration with the baseline recorded on the 29/11/2017 labelled 'WMI_2015_3B_6V_3U_3R_4Y' from the baseline recorded on the 'TBA date' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
- Run performance check 'CST' - ensure it is performance check using bead lot 70466.#70466
- Once completed, there should be an error detected with the UV laser. Click ok OK and close the CST window.
- Create a new experiment.
- Apply application settings. Note the application settings applied should have been created between 29/11/2017 and 'TBA date'.
- Record all voltages using a screen shot.
- Switch to the instrument configuration with the baseline recorded on the 'TBA date' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
- Create a new experiment, enter the voltages from the screen capture into the cytometer settings.
- Save these application settings as updated application settings that will now be linked with the baseline recorded on 'TBA date' labelled 'WMI_2018_3B_6V_3U_3R_4Y'
- Proceed as normal applying the updated application settings to your experiments.
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