Centrifuging fluorescent antibodies prior to using them may remove aggregates resulting in cleaner data

Sometimes you may notice a number of events above the obvious positive population, shown below. On occasion these may be fluorescent molecule aggregations that are super bright. 1 trick (and I hope to upload a before and after example when I dig it up) is to either individually centrifuge antibodies before using them, or centrifuging the cocktail before adding to cells in order to remove fluorescent aggregates. In a micro-centrifuge, 10000-12000g, chilled, 5 minutes can be used to pellet aggregates, followed by pipetting the supernatant.

Image Added

Ensure FSC-A, FSC-H, FSC-W & SSC-A, SSC-H, SSC-W are collected

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