This page is currently under construction - check back soon!

...

In FlowJo, after importing your cytometry data, click the 'Check sample quality' button in the tools work space. This will identify any samples that should be checked. FlowJo plots the median values for each parameter over time and flags any that are outside of 2 standard deviations. Green is good. Any thing else should be reviewed. 

FlowJo Active Check Sample Quality Plugin Access

Active sample quality check - FlowAI

FlowJo also provides access to 2 active cytometry data quality plugins. FlowClean & FlowAI

...

• Have R installed (version >3.4)
• Install BioConductor
  • source("https://bioconductor.org/biocLite.R"
  • biocLite("flowCore")
• Install flowCore (copy and paste the following)
  • source("https://bioconductor.org/biocLite.R")
  • biocLite("flowCore")
• Install PNG package
  • install.packages("png")
• Install flowAI
  • source("https://bioconductor.org/biocLite.R")
  • biocLite("flowAI")
• Have FlowJo installed with the correct R path in the preferences
  
• Download and locate FlowAI.jar file into the FlowJo plugins folder. 
• Restart FlowJo
• Select sample and navigate to Plugins, FlowAI.

These can be accessed from the plugins menu.Active sample quality check - FlowClean

Active sample quality check - R

Data transformation explained