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In regards to the UV laser change, please note that you will need to adjust the review voltages for the parameters off the UV laser line as these will have inevitably have changed.
The current suggested workflow involves
- Applying application settings
- Acquiring beads and adjusting parameter voltages for detectors off the UV laser line to bring the positive population to a similar MFI (from beads previously recorded before the UV laser change).
- Saving new application settings.as per usual and reviewing UV signals.
- Recording compensation controls.
This is suggested as other parameters should have been adequately updated when application settings had been applied.
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We have currently decided not to redefine the baseline to minimise impact to users (and other reasons including the baseline being validated on a different configuration). We also recommend recording new compensation controls.Some more investigation is continuing and we will update again if needed.
20180530 - Is there any way for me to gain access to FlowJo?
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