HTS acquisition on the LSRII
Reminder: Data is deleted off the flow cytometry analysers after 14 days.
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A new HD display screen has been installed in the shared lab. The screen is viewable from the level 2 café area and has been installed to highlight features of the Scientific Platforms as well as screen educational content. Screening will begin next week so keep a lookout for content!
HTS acquisition on the LSRII
The LSRII is equipped with a high throughput auto sampler that can be utilised to automate sample acquisition from 96 and 384 well plates. The HTS system minimizes carryover, provides user definable mixing and sample introduction protocols and is excellent for high number of samples. WRHFlow now has a supply of sterile 96 well V bottom plates and lids available for purchase through PPMS for high throughput sample staining, washing, and acquisition on the LSRII. Sterile V bottom plates with lids cost $6 and can be purchased via PPMS. Training can be requested through PPMS.
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Please remember we recommend compensation controls to be made and acquired in a similar manner and in parallel with your samples. We also recommend running and recording calibration beads both before and after your acquisitions to not only show the instrument was stable throughout the acquisition but to provide target MFIs in the event that application settings cannot be used on subsequent days.
Tip – Understanding negative fluorescence values and baseline restore
You may find yourself thinking it odd to have negative fluorescence values being reported in flow data files when the absence of fluorescence on a particle, one may think, should equate to either a zero or a low positive value. This can however occur because the values reported are arbitrary measurements of fluorescence on cells due to digitization.
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