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#PBS -P 
#PBS -N BASE_processing
#PBS -l select=1:ncpus=2:ngpus=1:mem=32GB
#PBS -q=main
#PBS -l walltime=10:00:00

#PBS -q defaultQ

module load python/3.6.5
module load openmpi-gcc/3.1.3
module load cuda/10.0.130
module load mrtrix3tissue/5.2.7
module load singularity/3.5.3
module load fsl/6.0.3
module load ants/2.3.2
module load mrtrix3/3.0.1
module load freesurfer/6.0.0
source $FREESURFER_HOME/SetUpFreeSurfer.sh
export SUBJECTS_DIR=/scratch/Project/ProjectPreprocessingPreprocessing

# CODE FOR TERMINAL												                                                							                            
# list='XXX_XXX
# XXX_XXX
# XXX_XXX'							                                        
# for i in $list ; do qsub -v base=$i FS_BASE_Processing.pbs; done 

echo ${base}
cd $SUBJECTS_DIR

time_points=$(find ${SUBJECTS_DIR} -iname "${base}_MR*")
NumTPs=( $time_points )

if [[ ${#NumTPs[@]} = 2 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -all -openmp 4
elif [[ ${#NumTPs[@]} = 3 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -tp ${base}_MR3 -all -openmp 4
elif [[ ${#NumTPs[@]} = 4 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -tp ${base}_MR3 -tp ${base}_MR4 -all -openmp 4
elif [[ ${#NumTPs[@]} = 5 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -tp ${base}_MR3 -tp ${base}_MR4 -tp ${base}_MR5 -all -openmp 4
elif [[ ${#NumTPs[@]} = 6 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -tp ${base}_MR3 -tp ${base}_MR4 -tp ${base}_MR5 -tp ${base}_MR6 -all -openmp 4
elif [[ ${#NumTPs[@]} = 7 ]]
	then
	recon-all -base $base -tp ${base}_MR1 -tp ${base}_MR2 -tp ${base}_MR3 -tp ${base}_MR4 -tp ${base}_MR5 -tp ${base}_MR6 -tp ${base}_MR7 -all -openmp 4
else
	# if there is only one timepoint
	echo 'there is either only one timepoint (no longitudinal data) or there are more than 8, please edit the script."
fi

Check that the jobs have completed without error (use grep “Exit Status” * in the folder with your output files). Once all the BASE processing is completed you can submit the LONG processing for each timepoint using the script below. Again you need to fill in the Project name at the top and on the line with export SUBJECTS_DIR. When submitting the script, this time you will create a list of all time point IDs (ie Control_001_MR1).

#PBS -P 
#PBS -N LONG_processing
#PBS -l select=1:ncpus=2:mem=32GB
#PBS -q=main
#PBS -l walltime=10:00:00

#PBS -q defaultQ

module load python/3.6.5
module load openmpi-gcc/3.1.3
module load cuda/10.0.130
module load mrtrix3tissue/5.2.7
module load singularity/3.5.3
module load fsl/6.0.3
module load ants/2.3.2
module load mrtrix3/3.0.1
module load freesurfer/6.0.0
source $FREESURFER_HOME/SetUpFreeSurfer.sh
export SUBJECTS_DIR=/scratch/Project/Preprocessing

# CODE FOR TERMINAL												                                                							                            
# list='XXX_XXX_MRX
# XXX_XXX_MRX
# XXX_XXX_MRX'							                                        
# for i in $list ; do qsub -v base=$i FS_LONG_Processing.pbs; done

cd $SUBJECTS_DIR

echo ${ses}
base=${ses%????}
recon-all -long ${ses} ${base} -all -openmp 4

Again check that the jobs have completed without error (use grep “Exit Status” * in the folder with your output files). You can also have a look at your data directory to make sure there is now a folder for each BASE id and long folder for each time point (called TIMEPOINTID.long.BASEID).

Next, follow the instructions on the FreeSurfer wiki for Inspecting Longitudinal Data to check some of your data to make sure the pipeline is doing a good job.

*Note, The next steps depend on the design of your study. If you want to look at trajectories for individual groups (not comparing groups) you will want to create separate longitudinal qdec tables at this point and then repeat the statistical analysis steps for each group. If you would like to compare the groups in your model, include all the cases and add grouping columns. /wiki/spaces/FN/pages/2831319107, so you may need to request access to Argus. Alternatively you can follow the steps from the FreeSurfer Wiki on a local installation of MATLAB.*

To get the longitudinal data ready for statistical analysis (LinearMixedEffectsModels) you need to create a table (space separated as a text file) in the following format:

where the first column is called fsid (containing all time points of all subjects) and the second column is fsid-base containing the within-subject template (=base) name, to group time points within subject. You can have many more columns such as gender, age, group, etc. Make sure you include the yearsfromBL column with an accurate measure of the time from the baseline scan for each time point (NOT the time from the previous timepoint). You can use the excel formula (=YEARFRAC) to get the time difference as a year fraction. If you are interested in including other variables in the model, add them into your longitudinal QDEC table. Make sure there are no spaces in the column headings as the file is space separated.