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QX200 Digital Droplet PCR

Protocol for Evagreen Supermix 

Includes:

  • DNA input requirements

  • Restriction digestion recommendations

  • Reaction setup

  • Thermal cycling conditions

Protocol for Probe Supermix 

Includes:

  • DNA input requirements

  • Restriction digestion recommendations

  • Reaction setup

  • Thermal cycling conditions

Nanostring 

Wet lab protocols

nCounter XT Assay Protocol 

Includes assay description and protocols for:

  • nCounter XT Codeset gene expression assay
  • nCounter XT Plex2  expression assay
  • nCounter Vantage RNA assay
  • nCounter Vantage RNA:Protein assay
  • nCounter XT DNA assay
  • nCounter XT gene expression assays for single cells and low input material
  • nCounter Vantage Fusion assay

nCounter Elements XT Reagents 

Includes assay description and protocols for:

  • nCounter Elements XT gene expression assay
  • nCounter Elements XT fusion gene assay
  • nCounter Elements XT DNA assay

nCounter miRNA Expression Assay Protocol 

Includes assay description and protocol for:

  • miRNA expression assay

Next Generation Sequencing

Illumina Library preparation protocols

DNA sequencing

RNA sequencing

10X Genomics Chromium Single Cell RNA-seq

Tapestation

Protocols

Short Tandem Repeat (STR) Cell Line Authentication

...

All protocols used by the genomics facility are provided in this section.